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PLA2G6 Antibody (CF803198) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLA2G6 (RC208735, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PLA2G. Positive lysates LY400377 (100 µg) and LC400377 (20 µg) can be purchased separately from OriGene.
Product Details
CF803198
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI10A2
Immunogen
Human recombinant protein fragment corresponding to amino acids 490-752 of human PLA2G6 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only two of them have been determined to date.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-lysophosphatidylcholine acylhydrolase; 85 kDa calcium-independent phospholipase A2; 85/88 kDa calcium-independent phospholipase A2; CaI-PLA2; Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; neurodegeneration with brain iron accumulation 2; Palmitoyl-CoA hydrolase; Patatin-like phospholipase domain-containing protein 9; phospholipase A2, group VI (cytosolic, calcium-independent); PNPLA9
Gene Aliases: CaI-PLA2; GVI; INAD1; iPLA2; IPLA2-VIA; iPLA2beta; NBIA2; NBIA2A; NBIA2B; PARK14; PLA2; PLA2G6; PLPLA9; PNPLA9
UniProt ID: (Human) O60733
Entrez Gene ID: (Human) 8398
positive regulation of protein phosphorylation
chemotaxis
positive regulation of cytosolic calcium ion concentration
memory
urinary bladder smooth muscle contraction
lipid catabolic process
antibacterial humoral response
cardiolipin biosynthetic process
response to endoplasmic reticulum stress
positive regulation of insulin secretion involved in cellular response to glucose stimulus
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
Fc-gamma receptor signaling pathway involved in phagocytosis
positive regulation of vasodilation
positive regulation of exocytosis
negative regulation of synaptic transmission, glutamatergic
maternal process involved in female pregnancy
positive regulation of protein kinase C signaling
positive regulation of release of cytochrome c from mitochondria
positive regulation of arachidonic acid secretion
regulation of store-operated calcium channel activity
positive regulation of ceramide biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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