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PLB1 Antibody (PA5-120091) in WB
Western blot analysis of PLB1 in extracts of various cell lines. Samples were incubated with PLB1 Polyclonal antibody (Product # PA5-120091) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-120091
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1110-1415 of human PLB1 (NP_694566.4).
Conjugate
Form
Liquid
Concentration
1.56 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913663
Product Specific Information
Positive test controls include: A-431, U-87MG, HL-60, HT-29, Mouse testis, Mouse lung, Mouse skeletal muscle, Rat testis. The target is usually found in the following locations: Apical cell membrane, Single-pass type I membrane protein.
Immunogen sequence: ARPNNSSDLP TSWRGLSWSI GGDGNLETHT TLPNILKKFN PYLLGFSTST WEGTAGLNVA AEGARARDMP AQAWDLVERM KNSPDINLEK DWKLVTLFIG VNDLCHYCEN PEAHLATEYV QHIQQALDIL SEELPRAFVN VVEVMELASL YQGQGGKCAM LAAQNNCTCL RHSQSSLEKQ ELKKVNWNLQ HGISSFSYWH QYTQREDFAV VVQPFFQNTL TPLNERGDTD LTFFSEDCFH FSDRGHAEMA IALWNNMLEP VGRKTTSNNF THSRAKLKCP SPESPYLYTL RNSRLLPDQA EEAPEV
Target Information
This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Lysophospholipase; Phospholipase A2; Phospholipase B; Phospholipase B/lipase; Phospholipase B1, membrane-associated; PLB/LIP; Triacylglycerol lipase
Gene Aliases: 4632413E21Rik; 4930433E17Rik; 4930539A06Rik; BC033606; hPLB; Phlpb; PLB; PLB/LIP; PLB1
UniProt ID: (Human) Q6P1J6, (Rat) O54728, (Mouse) Q3TTY0
Entrez Gene ID: (Human) 151056, (Rat) 192259, (Mouse) 665270
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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