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PLD2 Antibody (PA5-23335) in IHC (P)
Immunohistochemical analysis of PLD2 in formalin fixed paraffin-embedded (FFPE) human colon cancer. Samples were incubated in PLD2 polyclonal antibody (Product # PA5-23335) using a dilution of 1:100. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. ... View More
Product Details
PA5-23335
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Within the range amino acids 199-230 of human PLD2 protein
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum with 50% glycerol
Contains
0.025% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2540859
Target Information
PLD2 is a peripheral membrane protein belonging to phospholipase D family with a PH domain, two PLD phosphodiesterase domains and a PX (phox homology) domain that plays a regulatory rather than catalytic role. PLD2 catalyzes the hydrolysis of phosphatidylcholine (PC) to produce phosphatidic acid and choline. PLD2 may function in regulated secretion, signal-induced cytoskeletal regulation and/or endocytosis, transcriptional regulation, and cell cycle control. It is usually stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and activated by the ADP-ribosylation factor-1 (ARF-1). PLD2 is activated by agonist stimulation of both tyrosine kinase and G protein-coupled receptors and is also known to interact with EGFR and PIP5K1A. PLD2, localized to plasma membrane caveolae, is ubiquitously expressed in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Choline phosphatase 2; hPLD2; Phosphatidylcholine-hydrolyzing phospholipase D2; Phospholipase D2; PLD 2; PLD1C
Gene Aliases: PLD2
UniProt ID: (Human) O14939
Entrez Gene ID: (Human) 5338
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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