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PLEKHA5 Antibody (A304-599A) in IP
Detection of human PLEKHA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLEKHA5 antibody (Product # A304-599A) (lot 1) used for IP at 6 µg per reaction. PLEKHA5 was also immunoprecipitated by rabbit anti-PLEKHA5 antibody A304-600A. For blotting immunoprecipitated PLEKHA5, A304-599A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A304-599A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 875 to 925 of Human Pleckstrin Homology Domain Containing, Family A Member 5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PEPP-2; PH domain-containing family A member 5; Phosphoinositol 3-phosphate-binding protein 2; phosphoinositol 3-phosphate-binding protein-2; pleckstrin homology domain containing, family A member 5; Pleckstrin homology domain-containing family A member 5
Gene Aliases: KIAA1686; PEPP-2; PEPP2; PLEKHA5
UniProt ID: (Human) Q9HAU0
Entrez Gene ID: (Human) 54477
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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