Invitrogen

PLK1 Polyclonal Antibody

View all (67) PLK1 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-28023

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLK1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2545499

Product Specific Information

Recommended positive controls: A431, H1299, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (95%), Rat (95%), Chicken (80%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

View more View less

Gene Aliases: PLK; PLK1; STPK13

View more View less

UniProt ID: (Human) P53350, (Mouse) Q07832

View more View less

Entrez Gene ID: (Human) 5347, (Mouse) 18817

View more View less

Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding microtubule binding anaphase-promoting complex binding kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
mitotic sister chromatid segregation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis cytokinesis microtubule bundle formation protein phosphorylation cell cycle mitotic nuclear division mitotic spindle assembly checkpoint regulation of mitotic cell cycle positive regulation of peptidyl-threonine phosphorylation phosphorylation female meiosis chromosome segregation protein ubiquitination peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition G2 DNA damage checkpoint protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process polar body extrusion after meiotic divisions negative regulation of apoptotic process regulation of protein binding homologous chromosome segregation establishment of protein localization negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of proteolysis centrosome organization cell division positive regulation of ubiquitin-protein transferase activity synaptonemal complex disassembly protein localization to chromatin regulation of mitotic spindle assembly positive regulation of ubiquitin protein ligase activity sister chromatid cohesion mitotic nuclear envelope disassembly cell proliferation anaphase-promoting complex-dependent catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of cell cycle positive regulation of protein localization to nucleus regulation of cell cycle G2/M phase transition
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support