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Invitrogen
PLOD1 Polyclonal Antibody
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PLOD1 Antibody (PA5-89593) in WB
Western blot analysis of PLOD1 in extracts of Mouse heart. Samples were incubated with PLOD1 Polyclonal antibody (Product # PA5-89593) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Product Details
PA5-89593
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 488-727 of human PLOD1 (NP_0002932)
Conjugate
Form
Liquid
Concentration
1.21 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805676
Product Specific Information
Immunogen sequence: RQQDVFMFLT NRHTLGHLLS LDSYRTTHLH NDLWEVFSNP EDWKEKYIHQ NYTKALAGKL VETPCPDVYW FPIFTEVACD ELVEEMEHFG QWSLGNNKDN RIQGGYENVP TIDIHMNQIG FEREWHKFLL EYIAPMTEKL YPGYYTRAQF DLAFVVRYKP DEQPSLMPHH DASTFTINIA LNRVGVDYEG GGCRFLRYNC SIRAPRKGWT LMHPGRLTHY HEGLPTTRGT RYIAVSFVDP; Positive Samples: Mouse heart; Cellular Location: Lumenal side, Peripheral membrane protein, Rough endoplasmic reticulum membrane
Target Information
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LH1; Lysyl hydroxylase 1; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Gene Aliases: 2410042F05Rik; AI854890; AV007929; Lh1; Plod; Plod1
UniProt ID: (Mouse) Q9R0E2
Entrez Gene ID: (Mouse) 18822
iron ion binding
ferrous iron binding
procollagen-lysine 5-dioxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
L-ascorbic acid binding
peptide binding
metal ion binding
dioxygenase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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