Proteintech

PLOD3 Monoclonal Antibody (2G12B8)

PLOD3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

PLOD3 Antibody (60058-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using (Product # 60058-1-IG) (PLOD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

PLOD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLOD3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

60058-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G12B8

Immunogen

PLOD3 Fusion Protein Ag1480 (339-738 aa encoded by BC011674)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: HEPHIADSW PQLQDHFSAV KLVGPEEALS PGEARDMAMD LCRQDPECEF YFSLDADAVL TNLQTLRILI EENRKVIAPM LSRHGKLWSN FWGALSPDEY YARSEDYVEL VQRKRVGVWN VPYISQAYVI RGDTLRMELP QRDVFSGSDT DPDMAFCKSF RDKGIFLHLS NQHEFGRLLA TSRYDTEHLH PDLWQIFDNP VDWKEQYIHE NYSRALEGEG IVEQPCPDVY WFPLLSEQMC DELVAEMEHY GQWSGGRHED SRLAGGYENV PTVDIHMKQV GYEDQWLQLL RTYVGPMTES LFPGYHTKAR AVMNFVVRYR PDEQPSLRPH HDSSTFTLNV ALNHKGLDYE GGGCRFLRYD CVISSPRKGW ALLHPGRLTH YHEGLPTTWG TRYIMVSFVD P (339-738 aa encoded by BC011674)

Target Information

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis.

JCI insight

Tan X,Banerjee P,Liu X,Yu J,Lee S,Ahn YH,Creighton CJ,Kurie JM

Tue Jan 11 00:00:00 UTC 2022

Use of osteoblast-derived matrix to assess the influence of collagen modifications on cancer cells.

Matrix biology plus

Bota-Rabassedas N,Guo HF,Banerjee P,Chen Y,Terajima M,Yamauchi M,Kurie JM

Sun Nov 01 00:00:00 UTC 2020

Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa.

PloS one

Watt SA,Dayal JH,Wright S,Riddle M,Pourreyron C,McMillan JR,Kimble RM,Prisco M,Gartner U,Warbrick E,McLean WH,Leigh IM,McGrath JA,Salas-Alanis JC,Tolar J,South AP

Tue May 31 00:00:00 UTC 2016

Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa.

PloS one

Watt SA,Dayal JH,Wright S,Riddle M,Pourreyron C,McMillan JR,Kimble RM,Prisco M,Gartner U,Warbrick E,McLean WH,Leigh IM,McGrath JA,Salas-Alanis JC,Tolar J,South AP

Tue May 31 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: lysine hydroxylase 3; lysyl hydroxlase 3; lysyl hydroxylase 3; Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3; procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

View more View less

Gene Aliases: LH3; PLOD3

View more View less

UniProt ID: (Human) O60568

View more View less

Entrez Gene ID: (Human) 8985

View more View less

Function(s)

iron ion binding protein binding procollagen-lysine 5-dioxygenase activity L-ascorbic acid binding procollagen glucosyltransferase activity procollagen galactosyltransferase activity

Process(es)

in utero embryonic development endothelial cell morphogenesis protein localization neural tube development collagen fibril organization cellular response to hormone stimulus vasodilation epidermis morphogenesis oxidation-reduction process lung morphogenesis basement membrane assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PLOD3 Monoclonal Antibody (2G12B8)

PLOD3 Antibody (60058-1-IG) in ICC/IF

Product Details

60058-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!