Proteintech

PLSCR1 Polyclonal Antibody

PLSCR1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

PLSCR1 Antibody (11582-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PLSCR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLSCR1 Antibody in Flow Cytometry (Flow)

PLSCR1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11582-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PLSCR1 Fusion Protein Ag2172 (1-318 aa encoded by BC021100)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDKQNSQMN ASHPETNLPV GYPPQYPPTA FQGPPGYSGY PGPQVSYPPP PAGHSGPGPA GFPVPNQPVY NQPVYNQPVG AAGVPWMPAP QPPLNCPPGL EYLSQIDQIL IHQQIELLEV LTGFETNNKY EIKNSFGQRV YFAAEDTDCC TRNCCGPSRP FTLRIIDNMG QEVITLERPL RCSSCCCPCC LQEIEIQAPP GVPIGYVIQT WHPCLPKFTI QNEKREDVLK ISGPCVVCSC CGDVDFEIKS LDEQCVVGKI SKHWTGILRE AFTDADNFGI QFPLDLDVKM KAVMIGACFL IDFMFFESTG SQEQKSGVW (1-318 aa encoded by BC021100 )

Target Information

The calcium-dependent plasma membrane protein phospholipid scramblase 1 (PLSCR1) contributes to the transbilayer movement of phosphatidylserine and other membrane phospholipids upon influx of calcium into the cytosol. This movement results in plasma membrane phospholipid remodelling and surface exposure of phosphatidylserine in injured or apoptotic cells, which leads to cell death. Interferons and other cytokines induce expression of PLSCR1, implying that PLSCR1 also functions in cytokine signaling pathways. EGF stilmulation results in tyrosine phosphorylation of PLSCR1 on tyrosines 69 and 74, which allows it to interact with Shc, and thereby connecting Src kinase activation to stimulation of the EGF receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lungs via CRTH2-dependent mechanisms.

The Journal of clinical investigation

Hernandez-Gutierrez A,Majid S,Eberle A,Choi A,Sorkhdini P,Yang D,Yang AX,Norbrun C,He CH,Lee CM,Lee CG,Elias JA,Zhou Y

Tue Aug 01 00:00:00 UTC 2023

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lungs via CRTH2-dependent mechanisms.

The Journal of clinical investigation

Hernandez-Gutierrez A,Majid S,Eberle A,Choi A,Sorkhdini P,Yang D,Yang AX,Norbrun C,He CH,Lee CM,Lee CG,Elias JA,Zhou Y

Tue Aug 01 00:00:00 UTC 2023

ACKR1 favors transcellular over paracellular T-cell diapedesis across the blood-brain barrier in neuroinflammation in vitro.

European journal of immunology

Marchetti L,Francisco D,Soldati S,Haghayegh Jahromi N,Barcos S,Gruber I,Pareja JR,Thiriot A,von Andrian U,Deutsch U,Lyck R,Bruggmann R,Engelhardt B

Sat Jan 01 00:00:00 UTC 2022

Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.

Cancer science

Liao WC,Lin TJ,Liu YC,Wei YS,Chen GY,Feng HP,Chang YF,Chang HT,Wang CL,Chi HC,Wang CI,Lin KH,Ou Yang WT,Yu CJ

Sat Jan 01 00:00:00 UTC 2022

Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.

Cancer science

Liao WC,Lin TJ,Liu YC,Wei YS,Chen GY,Feng HP,Chang YF,Chang HT,Wang CL,Chi HC,Wang CI,Lin KH,Ou Yang WT,Yu CJ

Sat Jan 01 00:00:00 UTC 2022

Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.

Cancer science

Liao WC,Lin TJ,Liu YC,Wei YS,Chen GY,Feng HP,Chang YF,Chang HT,Wang CL,Chi HC,Wang CI,Lin KH,Ou Yang WT,Yu CJ

Sat Jan 01 00:00:00 UTC 2022

ILDR1 promotes influenza A virus replication through binding to PLSCR1.

Scientific reports