Invitrogen

PLZF Monoclonal Antibody (Mags.21F7), PE, eBioscience™

3 Published Figures

13 References

View all (18) PLZF antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

FIGURE: 1 / 4

PLZF Antibody (12-9320-82) in Flow

TOP: C57Bl/6 thymocytes were stained with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and Anti-Mouse TCR beta eFluor 450 (Product # 48-5961-82) followed by intracellular staining with 0.25 µg Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Mouse PLZF PE (right) using the Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). TCR beta+ cells in the lymphocyte gate were used for analysis. BOTTOM: C57Bl/6 thymocytes were stained with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and Anti-Mouse TCR beta eFluor 450 (... View More

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Product Details

12-9320-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Mags.21F7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11148934

Product Specific Information

Description: This Mags.21F7 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, and growth suppression. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.

Applications Reported: This Mags.21F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Mags.21F7 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse lymph node cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Transcriptional profiling of human Vδ1 T cells reveals a pathogen-driven adaptive differentiation program.

Cell reports

McMurray JL,von Borstel A,Taher TE,Syrimi E,Taylor GS,Sharif M,Rossjohn J,Remmerswaal EBM,Bemelman FJ,Vieira Braga FA,Chen X,Teichmann SA,Mohammed F,Berry AA,Lyke KE,Williamson KC,Stubbington MJT,Davey MS,Willcox CR,Willcox BE

12-9320-82 was used in Flow Cytometry to show that adult Vδ1+ T cells segregate into TCF7+LEF1+Granzyme Bneg (Tnaive) or T-bet+Eomes+BLIMP-1+Granzyme B+.

Tue May 24 00:00:00 UTC 2022

Loss of Ubiquitin Carboxy-Terminal Hydrolase L1 Impairs Long-Term Differentiation Competence and Metabolic Regulation in Murine Spermatogonial Stem Cells.

Cells

Alpaugh WF,Voigt AL,Dardari R,Su L,Al Khatib I,Shin W,Goldsmith TM,Coyle KM,Tang LA,Shutt TE,Klein C,Biernaskie J,Dobrinski I

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Tue Aug 31 00:00:00 UTC 2021

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Mon Dec 07 00:00:00 UTC 2020

Commensal Microbiota Promote Lung Cancer Development via γδ T Cells.

Cell

Jin C,Lagoudas GK,Zhao C,Bullman S,Bhutkar A,Hu B,Ameh S,Sandel D,Liang XS,Mazzilli S,Whary MT,Meyerson M,Germain R,Blainey PC,Fox JG,Jacks T

12-9320 was used in Flow cytometry/Cell sorting to link local microbiota-immune crosstalk to lung tumor development and thereby define key cellular and molecular mediators that may serve as effective targets in lung cancer intervention.

Thu Feb 21 00:00:00 UTC 2019

Transnuclear mice reveal Peyer's patch iNKT cells that regulate B-cell class switching to IgG1.

The EMBO journal

Clancy-Thompson E,Chen GZ,LaMarche NM,Ali LR,Jeong HJ,Crowley SJ,Boelaars K,Brenner MB,Lynch L,Dougan SK

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Mon Jul 15 00:00:00 UTC 2019

In Vitro Cytotoxicity of Folate-Silica-Gold Nanorods on Mouse Acute Lymphoblastic Leukemia and Spermatogonial Cells.

Cell journal

Eslahi N,Shakeri-Zadeh A,Ashtari K,Pirhajati-Mahabadi V,Tohidi Moghadam T,Shabani R,Kamrava K,Madjd Z,Maki C,Asgari HR,Koruji M

Published figure using PLZF monoclonal antibody (Product # 12-9320-82) in Flow Cytometry

Mon Apr 01 00:00:00 UTC 2019

Invariant natural killer T-cell development and function with loss of microRNA-155.

Immunology

Frias AB,Buechel HM,Neupane A,D'Cruz LM

12-9320 was used in Flow cytometry/Cell sorting to examine the role of microRNA-155 in the development and function of invariant natural killer T cells.

Thu Feb 01 00:00:00 UTC 2018

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

12-9320 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

Thu Mar 09 00:00:00 UTC 2017

MR1-restricted mucosal-associated invariant T (MAIT) cells respond to mycobacterial vaccination and infection in nonhuman primates.

Mucosal immunology

Greene JM,Dash P,Roy S,McMurtrey C,Awad W,Reed JS,Hammond KB,Abdulhaqq S,Wu HL,Burwitz BJ,Roth BF,Morrow DW,Ford JC,Xu G,Bae JY,Crank H,Legasse AW,Dang TH,Greenaway HY,Kurniawan M,Gold MC,Harriff MJ,Lewinsohn DA,Park BS,Axthelm MK,Stanton JJ,Hansen SG,Picker LJ,Venturi V,Hildebrand W,Thomas PG,Lewinsohn DM,Adams EJ,Sacha JB

12-9320 was used in Flow cytometry/Cell sorting to investigate the response of MR1 restricted mucosal-associated invariant T cells to mycobacterial vaccination and infection in nonhuman primates.

Mon May 01 00:00:00 UTC 2017

Reactive Oxygen Species Regulate the Inflammatory Function of NKT Cells through Promyelocytic Leukemia Zinc Finger.

Journal of immunology (Baltimore, Md. : 1950)

Kim YH,Kumar A,Chang CH,Pyaram K

12-9320 was used in Flow cytometry/Cell sorting to investigate the role of reactive oxygen species in natural killer T cell function.

Wed Nov 15 00:00:00 UTC 2017

The Hypothesis of the Human iNKT/Innate CD8(+) T-Cell Axis Applied to Cancer: Evidence for a Deficiency in Chronic Myeloid Leukemia.

Frontiers in immunology

Jacomet F,Cayssials E,Barbarin A,Desmier D,Basbous S,Lefèvre L,Levescot A,Robin A,Piccirilli N,Giraud C,Guilhot F,Roy L,Herbelin A,Gombert JM

Wed Nov 20 00:00:00 UTC 2019

Id2 regulates hyporesponsive invariant natural killer T cells.

Immunology and cell biology

Stradner MH,Cheung KP,Lasorella A,Goldrath AW,D'Cruz LM

12-9320 was used in Flow cytometry/Cell sorting to propose that downregulation of Id2 expression is essential to induction of the anti-inflammatory state in iNKT cells.

Mon Aug 01 00:00:00 UTC 2016

E and Id proteins influence invariant NKT cell sublineage differentiation and proliferation.

Journal of immunology (Baltimore, Md. : 1950)

D'Cruz LM,Stradner MH,Yang CY,Goldrath AW

12-9320 was used in Flow cytometry/Cell sorting to investigate the mechanism by which E and Id proteins control the development of invariant NKT sublineages after positive selection.

Sat Mar 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Green's luxoid; luxoid; promyelocytic leukaemia zinc finger; Promyelocytic leukemia zinc finger protein; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein PLZF

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Gene Aliases: AI467657; lu; PLZF; ZBTB16; Zfp145; ZNF145

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UniProt ID: (Human) Q05516

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Entrez Gene ID: (Human) 7704, (Mouse) 235320

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Function(s)

RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding protein binding protein C-terminus binding protein domain specific binding identical protein binding protein homodimerization activity metal ion binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter skeletal system development mesonephros development regulation of transcription, DNA-templated transcription from RNA polymerase II promoter central nervous system development negative regulation of cell proliferation embryonic pattern specification anterior/posterior pattern specification hemopoiesis embryonic limb morphogenesis positive regulation of chondrocyte differentiation protein localization to nucleus embryonic hindlimb morphogenesis forelimb morphogenesis hindlimb morphogenesis embryonic digit morphogenesis positive regulation of apoptotic process positive regulation of fat cell differentiation negative regulation of myeloid cell differentiation positive regulation of ossification negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated male germ-line stem cell asymmetric division positive regulation of NK T cell differentiation cartilage development positive regulation of cartilage development transcription, DNA-templated apoptotic process protein ubiquitination myeloid cell differentiation

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PLZF Monoclonal Antibody (Mags.21F7), PE, eBioscience™

PLZF Antibody (12-9320-82) in Flow

Product Details

12-9320-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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