Invitrogen

PLZF Monoclonal Antibody (Mags.21F7), Brilliant Violet™ 711, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

407-9320-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

1.0 µg/test
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 711, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Mags.21F7

Conjugate

Brilliant Violet™ 711 Brilliant Violet™ 711 Brilliant Violet™ 711

Excitation/Emission Max

406/711 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093245

Product Specific Information

Description: This Mags.21F7 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, and growth suppression. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.

Applications Reported: This Mags.21F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Mags.21F7 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Brilliant Violet™ 711 (BV711) is a tandem dye that emits at 713 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.

Our internal testing suggests that Brilliant Violet™ 711 (BV711) is not compatible with methanol-based fixation.

Excitation: 407 nm; Emission: 713 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Green's luxoid; luxoid; promyelocytic leukaemia zinc finger; Promyelocytic leukemia zinc finger protein; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein PLZF

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Gene Aliases: AI467657; lu; PLZF; ZBTB16; Zfp145; ZNF145

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UniProt ID: (Human) Q05516

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Entrez Gene ID: (Human) 7704, (Mouse) 235320

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Function(s)
RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding protein binding protein C-terminus binding protein domain specific binding identical protein binding protein homodimerization activity metal ion binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter skeletal system development mesonephros development regulation of transcription, DNA-templated transcription from RNA polymerase II promoter central nervous system development negative regulation of cell proliferation embryonic pattern specification anterior/posterior pattern specification hemopoiesis embryonic limb morphogenesis positive regulation of chondrocyte differentiation protein localization to nucleus embryonic hindlimb morphogenesis forelimb morphogenesis hindlimb morphogenesis embryonic digit morphogenesis positive regulation of apoptotic process positive regulation of fat cell differentiation negative regulation of myeloid cell differentiation positive regulation of ossification negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated male germ-line stem cell asymmetric division positive regulation of NK T cell differentiation cartilage development positive regulation of cartilage development transcription, DNA-templated apoptotic process protein ubiquitination myeloid cell differentiation
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