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Invitrogen
PMF1 Recombinant Rabbit Monoclonal Antibody (5X8X1)
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FIGURE: 1 / 2
PMF1 Antibody (MA5-55529) in WB
Western Blot analysis of various lysates using PMF1 Recombinant Monoclonal Antibody (Product # MA5-55529) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-55529
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5X8X1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human PMF1.
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667768
Product Specific Information
Immunogen Sequence: MAEASSANLG SGCEEKRHEG SSSESVPPGT TISRVKLLDT MVDTFLQKLV AAGSYQRFTD CYKCFYQLQP AMTQQIYDKF IAQLQTSIRE EISDIKEEGN LEAVLNALDK IVEEGKVRKE PAWRPSGIPE KDLHSVMAPY FLQQRDTLRR HVQKQEAENQ QLADAVLAGR RQVEELQLQV QAQQQAWQAL HREQRELVAV LREPE
Target Information
PMF1 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. It may act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Est1p-like protein B (EST1B); PMF-1; PMF1; Polyamine-modulated factor 1; RP11-54H19.4
Gene Aliases: 2600009M07Rik; AL033286; AW060657; PMF1
UniProt ID: (Human) Q6P1K2, (Mouse) Q9CPV5
Entrez Gene ID: (Human) 11243, (Rat) 681050, (Mouse) 67037
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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