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Invitrogen
PMPCB Monoclonal Antibody (9F13E4)
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FIGURE: 1 / 5
PMPCB Antibody (MA5-49303) in ICC/IF
Immunocytochemistry analysis of PMPCB in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PMPCB Monoclonal antibody (Product # MA5-49303) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
Product Details
MA5-49303
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
9F13E4
Immunogen
E.coli-derived human MPPB/PMPCB recombinant protein (Position: E23-Q479).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074961
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human HepG2 whole cell, human Hela whole cell, human HCCT tissue, rat liver tissue, rat RH35 whole cell, mouse liver tissue. IHC: human adenocarcinoma of lung tissue, human ovarian carcinoma tissue. ICC/IF: MCF-7 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
PMPCB is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-MPP; mitochondrial processing peptidase beta subunit; Mitochondrial-processing peptidase subunit beta; P-52; peptidase (mitochondrial processing) beta
Gene Aliases: 3110004O18Rik; Beta-MPP; MPP11; MPPB; MPPP52; P-52; PMPCB
UniProt ID: (Human) O75439, (Rat) Q03346, (Mouse) Q9CXT8
Entrez Gene ID: (Human) 9512, (Rat) 64198, (Mouse) 73078
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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