Proteintech

PNPT1 Polyclonal Antibody

PNPT1 Antibody in Immunocytochemistry (ICC/IF)

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PNPT1 Antibody (14487-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 14487-1-AP (PNPT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PNPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PNPT1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14487-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PNPT1 Fusion Protein Ag5888 (427-783 aa encoded by BC053660)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

Immunogen sequence: YEFPPYATN EIGKVTGLNR RELGHGALAE KALYPVIPRD FPFTIRVTSE VLESNGSSSM ASACGGSLAL MDSGVPISSA VAGVAIGLVT KTDPEKGEIE DYRLLTDILG IEDYNGDMDF KIAGTNKGIT ALQADIKLPG IPIKIVMEAI QQASVAKKEI LQIMNKTISK PRASRKENGP VVETVQVPLS KRAKFVGPGG YNLKKLQAET GVTISQVDEE TFSVFAPTPS AMHEARDFIT EICKDDQEQQ LEFGAVYTAT ITEIRDTGVM VKLYPNMTAV LLHNTQLDQR KIKHPTALGL EVGQEIQVKY FGRDPADGRM RLSRKVLQSP ATTVVRTLND RSSIVMGEPI SQSSSNSQ (427-783 aa encoded by BC053660)

Target Information

PNPT1 (polyribonucleotide nucleotidyltransferase 1, mitochondirla) is a RNA-binding protein implicated in numerous RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. PNPT1 is a component of the mitochondrial degradosome (mtEXO) complex that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. It is required for correct processing and polyadenylation of mitochondrial mRNAs. It plays a role as a cytoplasmic RNA import factor, in mitochondrial morphogenesis and respiration, regulation of expression of the electron transport chain, regulation of the stability of specific mature miRNAs in melanoma cells, and RNA cell surveilance. Mutations of the gene can result in combined oxidative phosphorylation deficiency 13 (COXPD13) and deafness, autosomal recessive, 70 (DFNB70).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pnpt1 mediates NLRP3 inflammasome activation by MAVS and metabolic reprogramming in macrophages.

Cellular & molecular immunology

Hsu CG,Li W,Sowden M,Chávez CL,Berk BC

Wed Feb 01 00:00:00 UTC 2023

Pnpt1 mediates NLRP3 inflammasome activation by MAVS and metabolic reprogramming in macrophages.

Cellular & molecular immunology

Hsu CG,Li W,Sowden M,Chávez CL,Berk BC

Wed Feb 01 00:00:00 UTC 2023

Pnpt1 mediates NLRP3 inflammasome activation by MAVS and metabolic reprogramming in macrophages.

Cellular & molecular immunology

Hsu CG,Li W,Sowden M,Chávez CL,Berk BC

Wed Feb 01 00:00:00 UTC 2023

Differences in Calcium Clearance at Inner Hair Cell Active Zones May Underlie the Difference in Susceptibility to Noise-Induced Cochlea Synaptopathy of C57BL/6J and CBA/CaJ Mice.

Frontiers in cell and developmental biology

Liu H,Peng H,Wang L,Xu P,Wang Z,Liu H,Wu H

Sat Feb 27 00:00:00 UTC 2021

RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer.

PLoS genetics

Majumder M,Palanisamy V

Wed Jan 01 00:00:00 UTC 2020

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

The EMBO journal

Szczepanowska K,Maiti P,Kukat A,Hofsetz E,Nolte H,Senft K,Becker C,Ruzzenente B,Hornig-Do HT,Wibom R,Wiesner RJ,Krüger M,Trifunovic A

Thu Dec 01 00:00:00 UTC 2016

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

The EMBO journal

Szczepanowska K,Maiti P,Kukat A,Hofsetz E,Nolte H,Senft K,Becker C,Ruzzenente B,Hornig-Do HT,Wibom R,Wiesner RJ,Krüger M,Trifunovic A

Thu Dec 01 00:00:00 UTC 2016

EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.

Proceedings of the National Academy of Sciences of the United States of America

Pangršič T,Gabrielaitis M,Michanski S,Schwaller B,Wolf F,Strenzke N,Moser T

Tue Mar 03 00:00:00 UTC 2015

EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.

Proceedings of the National Academy of Sciences of the United States of America

Pangršič T,Gabrielaitis M,Michanski S,Schwaller B,Wolf F,Strenzke N,Moser T

Tue Mar 03 00:00:00 UTC 2015

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.

American journal of human genetics

von Ameln S,Wang G,Boulouiz R,Rutherford MA,Smith GM,Li Y,Pogoda HM,Nürnberg G,Stiller B,Volk AE,Borck G,Hong JS,Goodyear RJ,Abidi O,Nürnberg P,Hofmann K,Richardson GP,Hammerschmidt M,Moser T,Wollnik B,Koehler CM,Teitell MA,Barakat A,Kubisch C

Fri Nov 02 00:00:00 UTC 2012

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.

American journal of human genetics

Fri Nov 02 00:00:00 UTC 2012

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.

American journal of human genetics

Fri Nov 02 00:00:00 UTC 2012

A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.

American journal of human genetics

Fri Nov 02 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 3'-5' RNA exonuclease OLD35; DKFZp762K1914; old 35; PNPase 1; PNPase old-35; Polynucleotide phosphorylase 1; Polynucleotide phosphorylase-like protein; Polyribonucleotide nucleotidyltransferase 1, mitochondrial

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Gene Aliases: 1200003F12Rik; COXPD13; DFNB70; old-35; OLD35; PNPASE; PNPT1; Pnptl1

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UniProt ID: (Human) Q8TCS8, (Mouse) Q8K1R3

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Entrez Gene ID: (Human) 87178, (Rat) 360992, (Mouse) 71701

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Function(s)

3'-5'-exoribonuclease activity polyribonucleotide nucleotidyltransferase activity protein binding poly(U) RNA binding poly(G) binding miRNA binding poly(A) RNA binding nucleic acid binding RNA binding nuclease activity exonuclease activity transferase activity nucleotidyltransferase activity hydrolase activity

Process(es)

mitochondrial RNA catabolic process mitochondrial mRNA catabolic process positive regulation of mitochondrial RNA catabolic process mitochondrial RNA 5'-end processing mitochondrial RNA 3'-end processing RNA catabolic process mRNA catabolic process cellular response to oxidative stress cellular response to interferon-beta RNA import into mitochondrion rRNA import into mitochondrion regulation of cellular respiration RNA polyadenylation negative regulation of growth protein homooligomerization positive regulation of mRNA catabolic process protein homotrimerization mitochondrion morphogenesis nuclear polyadenylation-dependent mRNA catabolic process mitotic cell cycle arrest RNA phosphodiester bond hydrolysis, exonucleolytic mitochondrial mRNA polyadenylation positive regulation of miRNA catabolic process regulation of cellular senescence RNA processing mRNA processing transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PNPT1 Polyclonal Antibody

PNPT1 Antibody (14487-1-AP) in ICC/IF

Product Details

14487-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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