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Invitrogen
POLD3 Polyclonal Antibody
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FIGURE: 1 / 4
POLD3 Antibody (PA5-96616) in ICC/IF
Immunofluorescence analysis of POLD3 in GFP-RNF168 transgenic U2OS cells. Samples were incubated with POLD3 Polyclonal antibody (Product # PA5-96616). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Product Details
PA5-96616
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_0065821)
Conjugate
Form
Liquid
Concentration
0.41 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808418
Product Specific Information
Immunogen sequence: ASKAAAKTQE TNKETKTEAK EVTNASAAGN KAPGKGNMMS NFFGKAAMNK FKVNLDSEQA VKEEKIVEQP TVSVTEPKLA TPAGLKKSSK KAEPVKVLQK EKKRGKRVAL SDDETKETEN MRKKRRRIKL PESDSSEDEV FPDSPGAYEA ESPSPPPPPS PPLEPVPKTE PEPPSVKSSS GENKRKRKRV LKSKTYLDGE GCIVTEKVYE SESCTDSEEE LNMKTSSVHR PPAMTVKKEP REERKGPKKG TAALGKANRQ VSITGFFQRK; Positive Samples: THP-1, HepG2, HeLa, MCF7, H460, Mouse brain; Cellular Location: Nucleus
Target Information
DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, exonucleases are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases, namely DNA pol alpha, beta, delta and epsilon. DNA pol delta and DNA pol epsilon are multisubunit enzymes, with DNA pol delta consisting of two subunits which interact with the sliding DNA clamp protein PCNA. DNA pol delta 3, also known as POLD3 or p66, is a 466 amino acid nuclear protein that is required for the proper function of DNA pol delta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA pol delta3; DNA polymerase delta subunit 3; DNA polymerase delta subunit C; DNA polymerase delta subunit p66; DNA polymerase delta subunit p68; DNApol delta3; GC12; Pol delta C subunit (p66); polymerase (DNA directed), delta 3; polymerase (DNA) delta 3, accessory subunit; polymerase (DNA-directed), delta 3, accessory subunit; protein phosphatase 1, regulatory subunit 128
Gene Aliases: 2410142G14Rik; C85233; KIAA0039; P66; P68; POLD3; PPP1R128
UniProt ID: (Human) Q15054
Entrez Gene ID: (Human) 10714, (Mouse) 67967
telomere maintenance via recombination
telomere maintenance
DNA synthesis involved in DNA repair
DNA strand elongation involved in DNA replication
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
translesion synthesis
nucleotide-excision repair, DNA incision
DNA damage response, detection of DNA damage
DNA replication
DNA-dependent DNA replication
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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