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Invitrogen
POLQ Polyclonal Antibody
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FIGURE: 1 / 1
POLQ Antibody (PA5-69577) in WB
Nature communications 2019 -
Fig. 1 Poltheta promotes efficient MMEJ of long ssDNA. a Schematic of Poltheta. b Schematic of MMEJ of DNA with long overhangs. c Schematic of Poltheta-pol ssDNAx via snap-back replication. d Schematic of Poltheta MMEJ of long ssDNA. e SDS gel (left) and Western blot (right) of purified human Poltheta. f Denaturing gel showing primer extension by Poltheta and Poltheta-pol. g TLC plate showing ... View More
Product Details
PA5-69577
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide directed towards the C terminal region of human POLQ
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2688722
Product Specific Information
This target displays homology in the following species: Cow: 93%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 86%; Rat: 100%
Target Information
DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA polymerase eta; DNA polymerase theta; EC 2.7.7.7; polymerase (DNA directed), theta; polymerase (DNA) theta
Gene Aliases: POLH; POLQ; PRO0327
UniProt ID: (Human) O75417
Entrez Gene ID: (Human) 10721
double-strand break repair via homologous recombination
DNA-dependent DNA replication
DNA repair
base-excision repair
double-strand break repair
cellular response to DNA damage stimulus
somatic hypermutation of immunoglobulin genes
protein homooligomerization
DNA biosynthetic process
double-strand break repair via alternative nonhomologous end joining
negative regulation of double-strand break repair via homologous recombination
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