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Product Details
H00010721-M09
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
1C11
Immunogen
POLQ (NP_006587, 2572 a.a. approximately 2680 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Sandwich ELISA (Recombinant protein).
Immunogen sequence: DGFVQTILGR RRYLPGIKDN NPYRKAHAER QAINTIVQGS AADIVKIATV NIQKQLETFH STFKSHGHRE GMLQSDRTGL SRKRKLQGMF CPIRGGFFIL QLHDELLYE
Target Information
DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA polymerase eta; DNA polymerase theta; EC 2.7.7.7; polymerase (DNA directed), theta; polymerase (DNA) theta
Gene Aliases: POLH; POLQ; PRO0327
UniProt ID: (Human) O75417
Entrez Gene ID: (Human) 10721
double-strand break repair via homologous recombination
DNA-dependent DNA replication
DNA repair
base-excision repair
double-strand break repair
cellular response to DNA damage stimulus
somatic hypermutation of immunoglobulin genes
protein homooligomerization
DNA biosynthetic process
double-strand break repair via alternative nonhomologous end joining
negative regulation of double-strand break repair via homologous recombination
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