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Invitrogen
POLR1B Polyclonal Antibody
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FIGURE: 1 / 1
POLR1B Antibody (PA5-88309) in WB
Western blot analysis of POLR1B in extracts of various cell lines. Samples were incubated with POLR1B Polyclonal antibody (Product # PA5-88309) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88309
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_0618872)
Conjugate
Form
Liquid
Concentration
0.65 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804819
Product Specific Information
Immunogen sequence: IANFIPFSDH NQSPRNMYQC QMGKQTMGFP LLTYQDRSDN KLYRLQTPQS PLVRPSMYDY YDMDNYPIGT NAIVAVISYT GYDMEDAMIV NKASWERGFA HGSVYKSEFI DLSEKIKQGD SSLVFGIKPG DPRVLQKLDD DGLPFIGAKL QYGDPYYSYL NLNTGESFVM YYKSKENCVV DNIKVCSNDT GSGKFKCVCI TMRVPRNPTI GDKFASRHGQ KGILSRLWPA E; Positive Samples: HeLa, HT-29, 293T, Mouse spleen; Cellular Location: Nucleus, nucleolus
Target Information
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; polymerase (RNA) I polypeptide B; polymerase (RNA) I polypeptide B, 128kDa; polymerase (RNA) I subunit B; RNA polymerase 1-2, 128 kDa subunit; RNA polymerase I subunit 2; RPA135
Gene Aliases: 128kDa; D630020H17Rik; POLR1B; RPA116; RPA135; RPA2; Rpo1-2
UniProt ID: (Human) Q9H9Y6, (Mouse) P70700
Entrez Gene ID: (Human) 84172, (Mouse) 20017
transcription, DNA-templated
embryo implantation
rRNA transcription
embryo development
nucleologenesis
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
positive regulation of gene expression, epigenetic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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