Proteintech

POLR1E Polyclonal Antibody

POLR1E Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

POLR1E Antibody (16145-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16145-1-AP ( POLR1E Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

POLR1E Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16145-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

POLR1E Fusion Protein Ag9269 (104-419 aa encoded by BC014331)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AELFNMQPL FSDVSVESEL ALESQTKTYR EKMDSCIEAF GTTKQKRALN TRRMNRVGNE SLNRAVAKAA ETIIDTKGVT ALVSDAIHND LQDDSLYLPP CYDDAAKPED VYKFEDLLSP AEYEALQSPS EAFRNVTSEE ILKMIEENSH CTFVIEALKS LPSDVESRDR QARCIWFLDT LIKFRAHRVV KRKSALGPGV PHIINTKLLK HFTCLTYNNG RLRNLISDSM KAKITAYVII LALHIHDFQI DLTVLQRDLK LSEKRMMEIA KAMRLKISKR KVSVAAGSEE DHKLGTLSLP LPPAQTSDRL AKRRKIT (104-419 aa encoded by BC014331 )

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors (PubMed:24207024). Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (PubMed:24207024). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancer.

Molecular biology of the cell

Rodrigues A,MacQuarrie KL,Freeman E,Lin A,Willis AB,Xu Z,Alvarez AA,Ma Y,White BEP,Foltz DR,Huang S

Sat Apr 01 00:00:00 UTC 2023

Expression of RNA polymerase I catalytic core is influenced by RPA12.

PloS one

Ford BL,Wei T,Liu H,Scull CE,Najmi SM,Pitts S,Fan W,Schneider DA,Laiho M

Fri Jun 02 00:00:00 UTC 2023

Identification of an E3 ligase that targets the catalytic subunit of RNA Polymerase I upon transcription stress.

The Journal of biological chemistry

Pitts S,Liu H,Ibrahim A,Garg A,Felgueira CM,Begum A,Fan W,Teh S,Low JY,Ford B,Schneider DA,Hay R,Laiho M

Thu Dec 01 00:00:00 UTC 2022

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

Science (New York, N.Y.)

Wu M,Xu G,Han C,Luan PF,Xing YH,Nan F,Yang LZ,Huang Y,Yang ZH,Shan L,Yang L,Liu J,Chen LL

Fri Jul 30 00:00:00 UTC 2021

Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains.

The Journal of biological chemistry

McNamar R,Abu-Adas Z,Rothblum K,Knutson BA,Rothblum LI

Fri Dec 27 00:00:00 UTC 2019

Quinacrine Depletes BCR-ABL and Suppresses Ph-Positive Leukemia Cells.

Cancer investigation

Lei H,Tu Y,Yang L,Jin J,Luo H,Xu H,Kang J,Zhou L,Wu Y

Fri Aug 02 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: DNA-directed RNA polymerase I subunit E; DNA-directed RNA polymerase I subunit RPA49; FLJ13390; FLJ13970; polymerase (RNA) I associated factor 1; polymerase (RNA) I polypeptide E; polymerase (RNA) I polypeptide E, 53kDa; polymerase (RNA) I subunit E; RNA polymerase I associated factor (Paf53); RNA polymerase I subunit A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53; RP11-405L18.3

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Gene Aliases: 53kDa; AU042259; D030019D19Rik; PAF53; POLR1E; PRAF1; RGD1565773

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UniProt ID: (Human) Q9GZS1, (Mouse) Q8K202

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Entrez Gene ID: (Human) 64425, (Mouse) 64424, (Rat) 313245

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Function(s)

RNA polymerase I activity RNA polymerase I transcription factor binding DNA binding DNA-directed RNA polymerase activity protein binding molecular_function

Process(es)

RNA polymerase I transcriptional preinitiation complex assembly at the promoter for the nuclear large rRNA transcript transcription, DNA-templated transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription rRNA transcription positive regulation of gene expression, epigenetic biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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POLR1E Polyclonal Antibody

POLR1E Antibody (16145-1-AP) in IHC (P)

Product Details

16145-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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