Proteintech

POLR2H Polyclonal Antibody

POLR2H Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

POLR2H Antibody (15086-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 15086-1-AP (POLR2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

POLR2H Antibody in Immunohistochemistry (Paraffin) (IHC (P))

POLR2H Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15086-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

POLR2H Fusion Protein Ag7166 (1-150 aa encoded by BC000739)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAGILFEDI FDVKDIDPEG KKFDRVSRLH CESESFKMDL ILDVNIQIYP VDLGDKFRLV IASTLYEDGT LDDGEYNPTD DRPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F (1-150 aa encoded by BC000739)

Target Information

Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S, and 5. 8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. The RPB6 and RPB8 subunits are shared by all 3 RNA polymerases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture.

Genome biology

Jiang Y,Huang J,Tian K,Yi X,Zheng H,Zhu Y,Guo T,Ji X

Mon Nov 28 00:00:00 UTC 2022

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity.

Molecular cell

Li Y,Huang J,Zhu J,Bao L,Wang H,Jiang Y,Tian K,Wang R,Zheng H,Duan W,Lai W,Yi X,Zhu Y,Guo T,Ji X

Thu Oct 20 00:00:00 UTC 2022

Identification of the key genes and pathways in prostate cancer.

Oncology letters

Fan S,Liang Z,Gao Z,Pan Z,Han S,Liu X,Zhao C,Yang W,Pan Z,Feng W

Thu Nov 01 00:00:00 UTC 2018

Identification of the key genes and pathways in prostate cancer.

Oncology letters

Fan S,Liang Z,Gao Z,Pan Z,Han S,Liu X,Zhao C,Yang W,Pan Z,Feng W

Thu Nov 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: ABC3; DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC3; hRPB8; hsRPB8; polymerase (RNA) II (DNA directed) polypeptide H; polymerase (RNA) II subunit H; RNA polymerases I, II, and III subunit ABC3; RPB17; RPB8 homolog

View more View less

Gene Aliases: POLR2H; RGD1561203; RPABC3; RPB17; RPB8

View more View less

UniProt ID: (Human) P52434, (Mouse) Q923G2

View more View less

Entrez Gene ID: (Human) 5437, (Mouse) 245841, (Rat) 498109

View more View less

Function(s)

RNA polymerase I activity RNA polymerase II activity RNA polymerase III activity DNA binding DNA-directed RNA polymerase activity

Process(es)

mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription from RNA polymerase III promoter fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA positive regulation of type I interferon production somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of gene expression, epigenetic positive regulation of viral transcription transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

POLR2H Polyclonal Antibody

POLR2H Antibody (15086-1-AP) in ICC/IF

Product Details

15086-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!