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PON3 Antibody (MA5-24173) in WB
Western blot analysis of PON3 in human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue. Samples were incubated in PON3 monoclonal antibody (Product # MA5-24173) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
MA5-24173
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
454929
Immunogen
E. coli-derived recombinant human PON3 Ala30-Leu354
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607774
Product Specific Information
This antibody detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON1 or rhPON2.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Paraoxonase-1 (PON1) is a calcium-dependent ester hydrolase that is tightly associated with apoA-I in HDL. PON1 prevents the oxidation of LDL, thereby the formation of atherogenic oxidised-LDL. Polymorphisms of the human PON1 gene are correlated with coronary artery disease, indicating a genetic association between PON1 and coronary artery disease. PON1 activity is lower in subjects prone to development of atherosclerosis such as in type 1 and type 2 diabetes, familial hypercholesterolemia, and renal disease, indicating the involvement of PON1 in atherosclerosis development. PON2 is a ubiquitously expressed intracellular protein that can protect cells against oxidative damage. PON3 is similar to PON1 in activity but differs from it in substrate specificity. PON1 and PON3 are implicated in lowering the risk of developing coronary artery disease and atherosclerosis. Human PON3 protein shares the 3 conserved cysteine residues identified in PON1, suggesting their importance of in vivo activities. PON1-based catalytic scavengers can be a way to safe and effective organophosphate intoxication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: arylesterase 3; paraoxanase-3; Serum paraoxonase/lactonase 3
Gene Aliases: 2810004E20; AI786302; PON3
UniProt ID: (Human) Q15166, (Rat) Q68FP2, (Mouse) Q62087
Entrez Gene ID: (Human) 5446, (Rat) 312086, (Mouse) 269823
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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