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POP5 Antibody (PA5-95789) in WB
Western blot analysis of POP5 in extracts of various cell lines. Samples were incubated with POP5 Polyclonal antibody (Product # PA5-95789) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-95789
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human POP5 (NP_0570022)
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807591
Product Specific Information
Immunogen sequence: SIGFAVRYLN AYTGIVLLRC RKEFYQLVWS ALPFITYLEN KGHRYPCFFN TLHVGGTIRT CQKFLIQYNR RQLLILLQNC TDEGEREAIQ KSVTRSCLLE E; Positive Samples: A-549, K562, 293T; Cellular Location: Nucleus, nucleolus
Target Information
Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:11413139, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hPop5; processing of precursor 5, ribonuclease P/MRP family; processing of precursor 5, ribonuclease P/MRP subunit; ribonuclease P protein 3; Ribonuclease P/MRP protein subunit POP5
Gene Aliases: AD-008; hPop5; HSPC004; POP5; Rnasep3; RPP2; RPP20; x0003
UniProt ID: (Human) Q969H6
Entrez Gene ID: (Human) 51367, (Rat) 117241
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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