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OriGene
POU4F3 Monoclonal Antibody (OTI4G7), TrueMAB™
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FIGURE: 1 / 1
POU4F3 Antibody (CF808728) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC21120°C, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F. Positive lysates LY400949 (100 µg) and LC400949 (20 µg) can be purchased separately from OriGene.
Product Details
CF808728
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4G7
Immunogen
Human recombinant protein fragment corresponding to amino acids 179-338 of human POU4F3 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
POU4F3 encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Brain-3C; brain-specific homeobox POU domain protein 3C; Brain-specific homeobox/POU domain protein 3C; Brn-3.1; brn-3C; MGC138412; POU domain, class 4, transcription factor 3
Gene Aliases: Brn-3.1; Brn-3c; Brn3.1; BRN3C; ddl; DFNA15; dreidel; POU4F3
UniProt ID: (Human) Q15319, (Rat) D3ZTL1, (Mouse) Q63955
Entrez Gene ID: (Human) 5459, (Rat) 364855, (Mouse) 18998
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
molecular_function
DNA binding
sequence-specific DNA binding
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
sensory perception of sound
biological_process
vestibulocochlear nerve development
retinal ganglion cell axon guidance
inner ear morphogenesis
auditory receptor cell differentiation
positive regulation of transcription from RNA polymerase II promoter
axon extension
inner ear development
neuromuscular process controlling balance
neuron apoptotic process
inner ear receptor cell differentiation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
visual perception
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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