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PP1 beta Antibody (PA1-12379) in ICC/IF
Immunofluorescence analysis of PPP1CB was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PP1 beta Polyclonal Antibody (Product # PA1-12379) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) ... View More
Product Details
PA1-12379
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
C-terminal peptide (amino acids 318-327) of PP1 beta catalytic subunit
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.08% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2170088
Product Specific Information
This antibody will not cross-react with other PP1 isoforms.
Target Information
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 80p; MGC21657; PP-1B; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX; protein phosphatase 1, catalytic subunit, beta isoform; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1, catalytic subunit, delta isoform; protein phosphatase 1-beta; protein phosphatase 1-delta; protein phosphatase-1a; serine/threonine protein phosphatase PP1-beta catalytic subunit; Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Gene Aliases: 1200010B19; BOS_11856; HEL-S-80p; PP-1B; PP1B; PP1beta; PPP1CB; PPP1CD
UniProt ID: (Human) P62140, (Bovine) Q3SWW9, (Mouse) P62141, (Rat) P62142
Entrez Gene ID: (Human) 5500, (Bovine) 538829, (Mouse) 19046, (Rat) 25594
G2/M transition of mitotic cell cycle
glycogen metabolic process
regulation of glycogen biosynthetic process
regulation of glycogen catabolic process
protein dephosphorylation
regulation of cell adhesion
circadian regulation of gene expression
regulation of circadian rhythm
entrainment of circadian clock by photoperiod
cell division
carbohydrate metabolic process
cell cycle
rhythmic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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