Invitrogen

PP2A alpha Polyclonal Antibody

View all (27) PP2A alpha antibodies

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Datasheet
Protocols
Questions & Answers

Product Details

PA5-95235

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807039

Product Specific Information

Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell, human Hela whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human colorectal adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: U2OS cell. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B55-GAMMA; IMYPNO; IMYPNO1; Methyl-PPP2CA; MGC33570; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; PP2A-alpha; PR52; PR55G; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase-2A-alpha; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

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Gene Aliases: PP2A; Pp2a1; PP2Ac; PP2CA; PP2Calpha; PPP2CA; R75353; RP-C

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UniProt ID: (Human) P67775, (Mouse) P63330, (Rat) P63331

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Entrez Gene ID: (Human) 5515, (Mouse) 19052, (Rat) 24672

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Function(s)
phosphoprotein phosphatase activity protein binding protein C-terminus binding metal ion binding GABA receptor binding protein serine/threonine phosphatase activity hydrolase activity enzyme binding protein kinase binding protein phosphatase binding protein domain specific binding protein complex binding protein kinase B binding ion channel binding protein dimerization activity tau protein binding protein phosphatase 2A binding protein antigen binding protein heterodimerization activity
Process(es)
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay inactivation of MAPK activity regulation of DNA replication regulation of transcription, DNA-templated protein dephosphorylation ceramide metabolic process apoptotic process mitotic nuclear envelope reassembly mesoderm development RNA splicing response to organic substance negative regulation of epithelial to mesenchymal transition second-messenger-mediated signaling regulation of Wnt signaling pathway regulation of cell adhesion negative regulation of cell growth regulation of growth negative regulation of tyrosine phosphorylation of Stat3 protein regulation of cell differentiation meiotic cell cycle positive regulation of protein serine/threonine kinase activity negative regulation of transcription from RNA polymerase II promoter regulation of protein phosphorylation negative regulation of protein phosphorylation regulation of receptor activity regulation of protein autophosphorylation positive regulation of protein phosphatase type 2A activity positive regulation of protein dephosphorylation regulation of protein catabolic process negative regulation of transcription factor import into nucleus positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cell cycle protein heterotrimerization cellular response to glucose stimulus negative regulation of calcium ion transmembrane transporter activity cardiac ventricle development heart development cellular response to insulin stimulus cellular response to calcium ion cellular response to cytokine stimulus cellular response to ethanol cellular response to follicle-stimulating hormone stimulus cellular response to steroid hormone stimulus
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