Rockland

PPAR alpha Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-421

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500- 1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:300
-

Immunocytochemistry (ICC/IF)

1-5 µg/mL
-

ELISA (ELISA)

1:75,000-1:125,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Bovine, Dog, Hamster, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-PPAR alpha Antibody is directed against mouse PPAR alpha protein. A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor alpha; Peroxisome proliferator-activated receptor alpha; PPAR alpha; PPAR-alpha

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Gene Aliases: 4933429D07Rik; AW742785; BOS_6232; I79_022392; NR1C1; PPAR; PPAR-alpha; PPARA; PPARALPHA

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UniProt ID: (Dog) Q95N78, (Rat) P37230, (Mouse) P23204

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Entrez Gene ID: (Pig) 397239, (Bovine) 281992, (Dog) 403654, (Rat) 25747, (Chinese hamster) 100758990, (Mouse) 19013

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding drug binding zinc ion binding lipid binding ubiquitin conjugating enzyme binding sequence-specific DNA binding protein binding phosphatase binding protein domain specific binding protein complex binding NFAT protein binding MDM2/MDM4 family protein binding metal ion binding protein heterodimerization activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to hypoxia transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter fatty acid metabolic process heart development epidermis development regulation of gene expression negative regulation of macrophage derived foam cell differentiation negative regulation of receptor biosynthetic process negative regulation of cholesterol storage negative regulation of sequestering of triglyceride regulation of fatty acid metabolic process intracellular receptor signaling pathway negative regulation of protein binding negative regulation of appetite response to insulin circadian regulation of gene expression behavioral response to nicotine wound healing lipoprotein metabolic process regulation of circadian rhythm steroid hormone mediated signaling pathway positive regulation of gluconeogenesis negative regulation of blood pressure positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fatty acid oxidation enamel mineralization regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter negative regulation of neuron death negative regulation of transcription regulatory region DNA binding glucose metabolic process lipid metabolic process rhythmic process
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