Invitrogen

PPAR alpha Polyclonal Antibody

PPAR alpha Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 35

PPAR alpha Antibody (PA1-822A) in IHC

Antioxidants (Basel, Switzerland) 2022 - Ppara was decreased in Ang II-induced vascular remodeling. ( A ) mRNA levels of Ppara in aortas of Ang II infusion WT mice compared with that of saline infusion WT mice ( n = 5-6, unpaired t -test, * p < 0.05). ( B ) Immunohistochemistry staining for PPARalpha in aortas of Ang II infusion mice and saline infusion mice. ( C ) Immunohistochemistry analysis of PPARalpha protein level ( n = 4, unpaired t -t... View More

PPAR alpha Antibody in Western Blot (WB)

PPAR alpha Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

PPAR alpha Antibody in Immunocytochemistry (ICC/IF)

PPAR alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)

PPAR alpha Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

PA1-822A

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 33 publications 33 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Avian, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2165595

Product Specific Information

PA1-822A detects peroxisome proliferator activated receptor (PPAR) alpha from mouse samples.

PA1-822A has been successfully used in Western blot, immunoprecipitation and immunofluoresence procedures. By Western blot, this antibody detects an ~52 kDa protein representing PPAR alpha from 3T3-L1 cell lysate. Immunofluoresence staining is observed predominantly in the nucleus as well as the cytoplasm.

PA1-822A immunogen is a synthetic peptide corresponding to residues M(1) V D T E S P I C P L S P L E A D D(18) C of mouse PPAR alpha. A single amino acid substitution (I8 to L8) exists between the mouse and human protein. This immunizing peptide (Cat.# PEP-025) is available for use in neutralization and control experiments.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peroxisome proliferator-activated receptor-α activation facilitates contextual fear extinction and modulates intrinsic excitability of dentate gyrus neurons.

Translational psychiatry

Xiang G,Liu X,Wang J,Lu S,Yu M,Zhang Y,Sun B,Huang B,Lu XY,Li X,Zhang D

PA1-822A was used in Western Blotting to provide evidence that PPARα plays an important role in regulating DG neuronal excitability and contextual fear extinction.

Thu Jun 15 00:00:00 UTC 2023

Peroxisome proliferator-activated receptor-α activation facilitates contextual fear extinction and modulates intrinsic excitability of dentate gyrus neurons.

Translational psychiatry

Xiang G,Liu X,Wang J,Lu S,Yu M,Zhang Y,Sun B,Huang B,Lu XY,Li X,Zhang D

PA1-822A was used in Western Blotting to provide evidence that PPARα plays an important role in regulating DG neuronal excitability and contextual fear extinction.

Thu Jun 15 00:00:00 UTC 2023

Sex Differences in Cardiac Pathology of SARS-CoV2 Infected and Trypanosoma cruzi Co-infected Mice.

Frontiers in cardiovascular medicine

Dhanyalayam D,Thangavel H,Lizardo K,Oswal N,Dolgov E,Perlin DS,Nagajyothi JF

PA1-822A was used in Western Blotting to conclude that the C-ApN/AMPK and b-AR/PPAR downstream signaling may play major roles in determining the progression, severity, and phenotype of CCM and heart failure in the context of COVID.

Tue Nov 15 00:00:00 UTC 2022

Sex Differences in Cardiac Pathology of SARS-CoV2 Infected and Trypanosoma cruzi Co-infected Mice.

Frontiers in cardiovascular medicine

Dhanyalayam D,Thangavel H,Lizardo K,Oswal N,Dolgov E,Perlin DS,Nagajyothi JF

Tue Nov 15 00:00:00 UTC 2022

Peroxisome Proliferator-Activated Receptor α Attenuates Hypertensive Vascular Remodeling by Protecting Vascular Smooth Muscle Cells from Angiotensin II-Induced ROS Production.

Antioxidants (Basel, Switzerland)

Liu Y,Duan Y,Zhao N,Zhu X,Yu X,Jiao S,Song Y,Shi L,Ma Y,Wang X,Yu B,Qu A

PA1-822A was used in Immunohistochemistry (Frozen) to suggest that PPARα plays a critical protective role in Ang II-induced hypertension via attenuating ROS production in VSMCs, thus providing a potential therapeutic target for hypertensive diseases.

Wed Nov 30 00:00:00 UTC 2022

Peroxisome Proliferator-Activated Receptor α Attenuates Hypertensive Vascular Remodeling by Protecting Vascular Smooth Muscle Cells from Angiotensin II-Induced ROS Production.

Antioxidants (Basel, Switzerland)

Liu Y,Duan Y,Zhao N,Zhu X,Yu X,Jiao S,Song Y,Shi L,Ma Y,Wang X,Yu B,Qu A

Wed Nov 30 00:00:00 UTC 2022

Differences in lipid metabolism in acquired versus preexisting glucose intolerance during gestation: role of free fatty acids and sphingosine-1-phosphate.

Lipids in health and disease

Liebmann M,Grupe K,Asuaje Pfeifer M,Rustenbeck I,Scherneck S

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Immunohistochemistry

Sat Oct 08 00:00:00 UTC 2022

Transcriptional Integration of Distinct Microbial and Nutritional Signals by the Small Intestinal Epithelium.

Cellular and molecular gastroenterology and hepatology

Lickwar CR,Davison JM,Kelly C,Mercado GP,Wen J,Davis BR,Tillman MC,Semova I,Andres SF,Vale G,McDonald JG,Rawls JF

PA1-822A was used in Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry to compare adult mice reared germ-free or conventionalized with a microbiota either fed normally or after a single high-fat meal.

Mon Jul 25 00:00:00 UTC 2022

PPAR-α Agonist Fenofibrate Ameliorates Sjögren Syndrome-Like Dacryoadenitis by Modulating Th1/Th17 and Treg Cell Responses in NOD Mice.

Investigative ophthalmology & visual science

Guo X,Dang W,Li N,Wang Y,Sun D,Nian H,Wei R

PA1-822A was used in Western Blotting to show that fenofibrate potently attenuated LG inflammation in a model of autoimmune dry eye, and this effect might partially result from regulating Th1/Th17/Treg cell responses by activating PPAR-α/LXR-β signaling.

Wed Jun 01 00:00:00 UTC 2022

Early-Life Exposure to Low-Dose Cadmium Accelerates Diethylnitrosamine and Diet-Induced Liver Cancer.

Oxidative medicine and cellular longevity

Men H,Young JL,Zhou W,Zhang H,Wang X,Xu J,Lin Q,Tan Y,Zheng Y,Cai L

PA1-822A was used in Western Blotting to investigate the impact of early-life exposure to cadmium on the incidence and potential mechanisms of hepatocellular carcinoma (HCC) in offspring subjected to postweaning HCC induction.

Tue Feb 22 00:00:00 UTC 2022

Early-Life Exposure to Low-Dose Cadmium Accelerates Diethylnitrosamine and Diet-Induced Liver Cancer.

Oxidative medicine and cellular longevity

Men H,Young JL,Zhou W,Zhang H,Wang X,Xu J,Lin Q,Tan Y,Zheng Y,Cai L

Tue Feb 22 00:00:00 UTC 2022

ACOX2 is a prognostic marker and impedes the progression of hepatocellular carcinoma via PPARα pathway.

Cell death & disease

Zhang Q,Zhang Y,Sun S,Wang K,Qian J,Cui Z,Tao T,Zhou J

PA1-822A was used in Western Blot to demonstrate a PRG ACOX2, and its overexpression reduced the proliferation and metastasis of liver cancer in vitro and in vivo through PPARα pathway.

Mon Jan 04 00:00:00 UTC 2021

ACOX2 is a prognostic marker and impedes the progression of hepatocellular carcinoma via PPARα pathway.

Cell death & disease

Zhang Q,Zhang Y,Sun S,Wang K,Qian J,Cui Z,Tao T,Zhou J

PA1-822A was used in Western Blot to demonstrate a PRG ACOX2, and its overexpression reduced the proliferation and metastasis of liver cancer in vitro and in vivo through PPARα pathway.

Mon Jan 04 00:00:00 UTC 2021

Molecular and phenotypic analysis of rodent models reveals conserved and species-specific modulators of human sarcopenia.

Communications biology

Börsch A,Ham DJ,Mittal N,Tintignac LA,Migliavacca E,Feige JN,Rüegg MA,Zavolan M

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Fri Feb 12 00:00:00 UTC 2021

Simiao Decoction Alleviates Gouty Arthritis by Modulating Proinflammatory Cytokines and the Gut Ecosystem.

Frontiers in pharmacology

Lin X,Shao T,Huang L,Wen X,Wang M,Wen C,He Z

PA1-822A was used in Western Blotting to suggest that Simiao decoction is an effective therapeutic drug for gouty arthritis and the gut ecosystem might act as a potential anti-inflammatory target of Simiao decoction.

Mon Sep 28 00:00:00 UTC 2020

Simiao Decoction Alleviates Gouty Arthritis by Modulating Proinflammatory Cytokines and the Gut Ecosystem.

Frontiers in pharmacology

Lin X,Shao T,Huang L,Wen X,Wang M,Wen C,He Z

Mon Sep 28 00:00:00 UTC 2020

Satellite glial cells promote regenerative growth in sensory neurons.

Nature communications

Avraham O,Deng PY,Jones S,Kuruvilla R,Semenkovich CF,Klyachko VA,Cavalli V

PA1-822A was used in Immunohistochemistry to reveal that satellite glial cellsare distinct from Schwann cells and share similarities with astrocytes.

Tue Sep 29 00:00:00 UTC 2020

Satellite glial cells promote regenerative growth in sensory neurons.

Nature communications

Avraham O,Deng PY,Jones S,Kuruvilla R,Semenkovich CF,Klyachko VA,Cavalli V

PA1-822A was used in Immunohistochemistry to reveal that satellite glial cellsare distinct from Schwann cells and share similarities with astrocytes.

Tue Sep 29 00:00:00 UTC 2020

Satellite glial cells promote regenerative growth in sensory neurons.

Nature communications

Avraham O,Deng PY,Jones S,Kuruvilla R,Semenkovich CF,Klyachko VA,Cavalli V

PA1-822A was used in Immunohistochemistry to reveal that satellite glial cellsare distinct from Schwann cells and share similarities with astrocytes.

Tue Sep 29 00:00:00 UTC 2020

Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth.

eLife

Contat C,Ancey PB,Zangger N,Sabatino S,Pascual J,Escrig S,Jensen L,Goepfert C,Lanz B,Lepore M,Gruetter R,Rossier A,Berezowska S,Neppl C,Zlobec I,Clerc-Rosset S,Knott GW,Rathmell JC,Abel ED,Meibom A,Meylan E

PA1-822A was used in Western Blotting to demonstrate the requirement for two glucose transporters in lung adenocarcinoma, the dual blockade of which could reach therapeutic responses not achieved by individual targeting.

Tue Jun 23 00:00:00 UTC 2020

Lysosomal inhibition attenuates peroxisomal gene transcription via suppression of PPARA and PPARGC1A levels.

Autophagy

Tan HWS,Anjum B,Shen HM,Ghosh S,Yen PM,Sinha RA

PA1-822A was used in Western Blotting to show PPARA-PPARGC1A transcriptional signaling promoted by lysosomes mediates metabolic regulation of peroxisomes.

Thu Aug 01 00:00:00 UTC 2019

Effect of liver fatty acid binding protein (L-FABP) gene ablation on lipid metabolism in high glucose diet (HGD) pair-fed mice.

Biochimica et biophysica acta. Molecular and cell biology of lipids

McIntosh AL,Atshaves BP,Martin GG,Landrock D,Milligan S,Landrock KK,Huang H,Storey SM,Mackie J,Schroeder F,Kier AB

PA1-822A was used in Western Blotting to investigate the potential impact high dietary glucose on the amount of L-FABP and on the whole-body and lipid phenotypes.

Mon Jul 01 00:00:00 UTC 2019

Diet Alters Serum Metabolomic Profiling in the Mouse Model of Chronic Chagas Cardiomyopathy.

Disease markers

Lizardo K,Ayyappan JP,Ganapathi U,Dutra WO,Qiu Y,Weiss LM,Nagajyothi JF

PA1-822A was used in Western Blot to present the results of serum metabolomic analyses and discuss its correlations to the diet-induced metabolic regulations in the pathogenesis of CCM in a murine model of Chagas disease.

Thu May 14 00:00:00 UTC 2020

Ginsenoside Rb1 Induces Beta 3 Adrenergic Receptor-Dependent Lipolysis and Thermogenesis in 3T3-L1 Adipocytes and db/db Mice.

Frontiers in pharmacology

Lim S,Park J,Um JY

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Sat Oct 14 00:00:00 UTC 2023

Fibrates as drugs with senolytic and autophagic activity for osteoarthritis therapy.

EBioMedicine

Nogueira-Recalde U,Lorenzo-Gómez I,Blanco FJ,Loza MI,Grassi D,Shirinsky V,Shirinsky I,Lotz M,Robbins PD,Domínguez E,Caramés B

PA1-822A was used in Western Blotting to demonstrate that fibrate drugs targeting lipid metabolism protect against cartilage degeneration seen with ageing and osteoarthritis.

Mon Jul 01 00:00:00 UTC 2019

Fenofibrate prevents skeletal muscle loss in mice with lung cancer.

Proceedings of the National Academy of Sciences of the United States of America

Goncalves MD,Hwang SK,Pauli C,Murphy CJ,Cheng Z,Hopkins BD,Wu D,Loughran RM,Emerling BM,Zhang G,Fearon DT,Cantley LC

PA1-822A was used in Immunoprecipitation to characterise the changes in systemic metabolism in a genetically engineered mouse model of non-small cell lung cancer.

Tue Jan 23 00:00:00 UTC 2018

Fenofibrate prevents skeletal muscle loss in mice with lung cancer.

Proceedings of the National Academy of Sciences of the United States of America

Goncalves MD,Hwang SK,Pauli C,Murphy CJ,Cheng Z,Hopkins BD,Wu D,Loughran RM,Emerling BM,Zhang G,Fearon DT,Cantley LC

PA1-822A was used in Immunoprecipitation to characterise the changes in systemic metabolism in a genetically engineered mouse model of non-small cell lung cancer.

Tue Jan 23 00:00:00 UTC 2018

Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Milligan S,Martin GG,Landrock D,McIntosh AL,Mackie JT,Schroeder F,Kier AB

PA1-822A was used in Western Blotting to study the effects of ablating both Fabp1 and Scp2/Scpx in high fat-fed mice.

Thu Mar 01 00:00:00 UTC 2018

Human Liver Fatty Acid Binding Protein-1 T94A Variant, Nonalcohol Fatty Liver Disease, and Hepatic Endocannabinoid System.

Lipids

Martin GG,Landrock D,Dangott LJ,McIntosh AL,Kier AB,Schroeder F

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Mon Jan 01 00:00:00 UTC 2018

Impact of dietary phytol on lipid metabolism in SCP2/SCPX/L-FABP null mice.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Milligan S,Martin GG,Landrock D,McIntosh AL,Mackie JT,Schroeder F,Kier AB

PA1822A was used in western blot to clarify the contributions of SCP2/SCPx gene ablation on dietary phytol-induced whole body and hepatic lipid phenotypes

Wed Mar 01 00:00:00 UTC 2017

Peroxisome Proliferator-Activated Receptor α Facilitates Osteogenic Differentiation in MC3T3-E1 Cells via the Sirtuin 1-Dependent Signaling Pathway.

Molecules and cells

Gong K,Qu B,Wang C,Zhou J,Liao D,Zheng W,Pan X

PA1-822A was used in Western Blotting to indicate that PPARα promotes osteogenic differentiation via the Sirt1-dependent signalling pathway.

Fri Jun 30 00:00:00 UTC 2017

Effect of Fabp1/Scp-2/Scp-x Ablation on Whole Body and Hepatic Phenotype of Phytol-Fed Male Mice.

Lipids

Landrock D,Milligan S,Martin GG,McIntosh AL,Landrock KK,Schroeder F,Kier AB

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Mon May 01 00:00:00 UTC 2017

PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.

Nature medicine

Dickey AS,Pineda VV,Tsunemi T,Liu PP,Miranda HC,Gilmore-Hall SK,Lomas N,Sampat KR,Buttgereit A,Torres MJ,Flores AL,Arreola M,Arbez N,Akimov SS,Gaasterland T,Lazarowski ER,Ross CA,Yeo GW,Sopher BL,Magnuson GK,Pinkerton AB,Masliah E,La Spada AR

PA1-822A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets

Fri Jan 01 00:00:00 UTC 2016

PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.

Nature medicine

PA1-822A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets

Fri Jan 01 00:00:00 UTC 2016

Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARα) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells.

Molecular and cellular endocrinology

Rotondi S,Modarelli A,Oliva MA,Rostomyan L,Sanita P,Ventura L,Daly AF,Esposito V,Angelucci A,Arcella A,Giangaspero F,Beckers A,Jaffrain-Rea ML

PA1-822A was used in immunohistochemistry - paraffin section and western blot to determine the relationship with aryl hydrocarbon receptor interacting protein (AIP) and in vitor effects of fenofibrate in GH3 cells to study expression of peroxisome prolif

Thu May 05 00:00:00 UTC 2016

Prenatal fat exposure and hypothalamic PPAR β/δ: Possible relationship to increased neurogenesis of orexigenic peptide neurons.

Peptides

Chang GQ,Karatayev O,Lukatskaya O,Leibowitz SF

Sun May 01 00:00:00 UTC 2016

Impact of SCP-2/SCP-x gene ablation and dietary cholesterol on hepatic lipid accumulation.

American journal of physiology. Gastrointestinal and liver physiology

Klipsic D,Landrock D,Martin GG,McIntosh AL,Landrock KK,Mackie JT,Schroeder F,Kier AB

PA1-822A was used in western blot to investigate the role of SCP-2/SCP-x and dietary cholesterol in hepatic lipid metabolism

Tue Sep 01 00:00:00 UTC 2015

Relative contributions of L-FABP, SCP-2/SCP-x, or both to hepatic biliary phenotype of female mice.

Archives of biochemistry and biophysics

Martin GG,Landrock D,Landrock KK,Howles PN,Atshaves BP,Kier AB,Schroeder F

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Tue Dec 15 00:00:00 UTC 2015

Loss of L-FABP, SCP-2/SCP-x, or both induces hepatic lipid accumulation in female mice.

Archives of biochemistry and biophysics

Martin GG,Atshaves BP,Landrock KK,Landrock D,Schroeder F,Kier AB

PA1-822A was used in western blot to investigate the role of L-FABP and SCP-2/SCP-x in hepatic lipid metabolism

Sat Aug 15 00:00:00 UTC 2015

Ablating L-FABP in SCP-2/SCP-x null mice impairs bile acid metabolism and biliary HDL-cholesterol secretion.

American journal of physiology. Gastrointestinal and liver physiology

Martin GG,Atshaves BP,Landrock KK,Landrock D,Storey SM,Howles PN,Kier AB,Schroeder F

PA1-822A was used in western blot to characterize ablation of L-FABP in SCP-2/SCP-x null mice and the impairment of bile acid metabolism and biliary HDL-cholesterol secretion

Mon Dec 01 00:00:00 UTC 2014

Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db mice, a model of type 2 diabetes mellitus.

The Journal of pharmacy and pharmacology

Bang CY,Choung SY

PA1-822A was used in western blot to study the mechanisms underlying the beneficial effects of a dietary pine bark extract on glucose tolerance in a murine model of type 2 diabetes

Sun Jun 01 00:00:00 UTC 2014

Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.

Journal of lipid research

Smathers RL,Galligan JJ,Shearn CT,Fritz KS,Mercer K,Ronis M,Orlicky DJ,Davidson NO,Petersen DR

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Immunoprecipitation

Wed May 01 00:00:00 UTC 2013

Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.

Journal of lipid research

Smathers RL,Galligan JJ,Shearn CT,Fritz KS,Mercer K,Ronis M,Orlicky DJ,Davidson NO,Petersen DR

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Immunoprecipitation

Wed May 01 00:00:00 UTC 2013

Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.

PPAR research

Huang H,McIntosh AL,Martin GG,Petrescu AD,Landrock KK,Landrock D,Kier AB,Schroeder F

PA1-822A was used in western blot to study the role of L-FABP and glucose in the induction of PPARalpha-regulated fatty acid beta-oxidation genes by fatty acid synthesis inhibitors

Thu Mar 28 00:00:00 UTC 2013

Liver fatty acid binding protein gene-ablation exacerbates weight gain in high-fat fed female mice.

Lipids

McIntosh AL,Atshaves BP,Landrock D,Landrock KK,Martin GG,Storey SM,Kier AB,Schroeder F

PA1-822A was used in western blot to study the increased weight gain in L-FABP-null female mice fed a high fat diet

Wed May 01 00:00:00 UTC 2013

Site-specific and time-dependent activation of the endocannabinoid system after transection of long-range projections.

PloS one

Kallendrusch S,Hobusch C,Ehrlich A,Ziebell S,Ueda N,Geisslinger G,Koch M,Dehghani F

PA1-822A was used in immunohistochemistry and western blot to study site-specific and time-dependent activation of the endocannabinoid system in response to neuronal damage

Fri Aug 03 00:00:00 UTC 2012

Site-specific and time-dependent activation of the endocannabinoid system after transection of long-range projections.

PloS one

Kallendrusch S,Hobusch C,Ehrlich A,Ziebell S,Ueda N,Geisslinger G,Koch M,Dehghani F

PA1-822A was used in immunohistochemistry and western blot to study site-specific and time-dependent activation of the endocannabinoid system in response to neuronal damage

Fri Aug 03 00:00:00 UTC 2012

Site-specific and time-dependent activation of the endocannabinoid system after transection of long-range projections.

PloS one

Kallendrusch S,Hobusch C,Ehrlich A,Ziebell S,Ueda N,Geisslinger G,Koch M,Dehghani F

PA1-822A was used in immunohistochemistry and western blot to study site-specific and time-dependent activation of the endocannabinoid system in response to neuronal damage

Fri Aug 03 00:00:00 UTC 2012

Reduction of body weight, liver steatosis and expression of stearoyl-CoA desaturase 1 by the isoflavone daidzein in diet-induced obesity.

British journal of pharmacology

Crespillo A,Alonso M,Vida M,Pavón FJ,Serrano A,Rivera P,Romero-Zerbo Y,Fernández-Llebrez P,Martínez A,Pérez-Valero V,Bermúdez-Silva FJ,Suárez J,de Fonseca FR

PA1-822A was used in immunohistochemistry to investigate the effect of isoflavone daidzein on rat obesity caused by high-fat diet

Thu Dec 01 00:00:00 UTC 2011

Palmitoylethanolamide protects dentate gyrus granule cells via peroxisome proliferator-activated receptor-α.

Neurotoxicity research

Koch M,Kreutz S,Böttger C,Benz A,Maronde E,Ghadban C,Korf HW,Dehghani F

PA1-822A was used in immunocytochemistry and western blot to investigate the mechanism of dentate gyrus granule cell protection mediated by palmitoylethanolamide

Tue Feb 01 00:00:00 UTC 2011

Regulation of the expression of the avian uncoupling protein 3 by isoproterenol and fatty acids in chick myoblasts: possible involvement of AMPK and PPARalpha?

American journal of physiology. Regulatory, integrative and comparative physiology

Joubert R,Métayer-Coustard S,Crochet S,Cailleau-Audouin E,Dupont J,Duclos MJ,Tesseraud S,Collin A

PA1-822A was used in western blot to investigate the effect of isoproterenol and fatty acids on avian uncoupling protein 3 and its mechanism

Fri Jul 01 00:00:00 UTC 2011

Palmitoylation of ketogenic enzyme HMGCS2 enhances its interaction with PPARalpha and transcription at the Hmgcs2 PPRE.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kostiuk MA,Keller BO,Berthiaume LG

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Tue Jun 01 00:00:00 UTC 2010

Palmitoylation of ketogenic enzyme HMGCS2 enhances its interaction with PPARalpha and transcription at the Hmgcs2 PPRE.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kostiuk MA,Keller BO,Berthiaume LG

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Tue Jun 01 00:00:00 UTC 2010

Palmitoylation of ketogenic enzyme HMGCS2 enhances its interaction with PPARalpha and transcription at the Hmgcs2 PPRE.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kostiuk MA,Keller BO,Berthiaume LG

Published figure using PPAR alpha polyclonal antibody (Product # PA1-822A) in Western Blot

Tue Jun 01 00:00:00 UTC 2010

PPARalpha and PPARgamma are co-expressed, functional and show positive interactions in the rat urinary bladder urothelium.

Journal of applied toxicology : JAT

Egerod FL,Brünner N,Svendsen JE,Oleksiewicz MB

PA1-822A was used in western blot to investigate the interaction between PPAR alpha and gamma in rat urinary bladder urothelium

Mon Mar 01 00:00:00 UTC 2010

Rapid non-genomic regulation of Ca2+ signals and insulin secretion by PPAR alpha ligands in mouse pancreatic islets of Langerhans.

The Journal of endocrinology

Ropero AB,Juan-Picó P,Rafacho A,Fuentes E,Bermúdez-Silva FJ,Roche E,Quesada I,de Fonseca FR,Nadal A

PA1-822A was used in immunocytochemistry to show the location of PPAR in insulin-containing cells

Sun Feb 01 00:00:00 UTC 2009

PPARalpha agonist fenofibrate suppresses tumor growth through direct and indirect angiogenesis inhibition.

Proceedings of the National Academy of Sciences of the United States of America

Panigrahy D,Kaipainen A,Huang S,Butterfield CE,Barnés CM,Fannon M,Laforme AM,Chaponis DM,Folkman J,Kieran MW

PA1-822A was used in western blot to investigate the suppressive effect of PPARalpha agonist fenofibrate on tumor growth and its mechanism

Tue Jan 22 00:00:00 UTC 2008

Urine acidification has no effect on peroxisome proliferator-activated receptor (PPAR) signaling or epidermal growth factor (EGF) expression in rat urinary bladder urothelium.

Toxicology and applied pharmacology

Achanzar WE,Moyer CF,Marthaler LT,Gullo R,Chen SJ,French MH,Watson LM,Rhodes JW,Kozlosky JC,White MR,Foster WR,Burgun JJ,Car BD,Cosma GN,Dominick MA

PA1-822A was used in immunohistochemistry to investigate the influence of urine acidification on signal pathways in urinary bladder urothelium

Sat Sep 15 00:00:00 UTC 2007

PPAR gamma activators induce growth arrest and process extension in B12 oligodendrocyte-like cells and terminal differentiation of cultured oligodendrocytes.

Journal of neuroscience research

Roth AD,Leisewitz AV,Jung JE,Cassina P,Barbeito L,Inestrosa NC,Bronfman M

PA1-822A was used in western blot to investigate the effect of PPARgamma activation on the growth and morphology of B12 oligodendrocyte-like cells

Thu May 15 00:00:00 UTC 2003

Nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha) is expressed in resting murine lymphocytes. The PPARalpha in T and B lymphocytes is both transactivation and transrepression competent.

The Journal of biological chemistry

Jones DC,Ding X,Daynes RA

PA1-822A was used in immunocytochemistry to investigate the expression of peroxisome proliferator-activated receptors in lymphocytes.

Fri Mar 01 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha

View more View less

Gene Aliases: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha

View more View less

UniProt ID: (Human) Q07869, (Mouse) P23204

View more View less

Entrez Gene ID: (Human) 5465, (Mouse) 19013

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription coactivator binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding drug binding zinc ion binding lipid binding phosphatase binding protein domain specific binding ubiquitin conjugating enzyme binding protein complex binding sequence-specific DNA binding NFAT protein binding MDM2/MDM4 family protein binding receptor activity metal ion binding protein heterodimerization activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PPAR alpha Polyclonal Antibody

PPAR alpha Antibody (PA1-822A) in IHC

Product Details

PA1-822A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!