Invitrogen

PPAR delta Polyclonal Antibody

PPAR delta Antibody in Immunocytochemistry (ICC/IF)

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PPAR delta Antibody (PA1-823A) in ICC/IF

Immunofluorescence analysis of PPAR delta was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PPAR delta Rabbit Polyclonal Antibody (Product # PA1-823A) at 1 µg/mL in 1% BSA and incubated for 3 hours at room tempe... View More

PPAR delta Antibody in Western Blot (WB)

PPAR delta Antibody in Immunohistochemistry (Frozen) (IHC (F))

PPAR delta Antibody in Immunohistochemistry (IHC)

PPAR delta Antibody in Western Blot, Immunohistochemistry (WB, IHC)

PPAR delta Antibody in Western Blot, Immunoprecipitation (WB, IP)

PPAR delta Antibody in ChIP Assay (ChIP)

Product Image Gallery

Product Details

PA1-823A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) E Q P Q E E T P E A R E E(14) C of mouse PPAR delta.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2165895

Product Specific Information

PA1-823A detects peroxisome proliferator activated receptor (PPAR) delta from rat, human and mouse cells. This antibody does not detect PPAR alpha or PPAR gamma.

PA1-823A has been successfully used in Western blot and immunohistochemical and immunofluoresence procedures. By Western blot, this antibody detects an ~49 kDa protein representing PPAR delta from NIH-3T3 cell lysate. Immunolocalization yeilds predominant nuclear staining.

The PA1-823A immunogen is a synthetic peptide corresponding to residues M(1) E Q P Q E E T P E A R E E(14) C of mouse PPAR delta. This sequence is 86% conserved in human PPAR delta. PA1-823A immunizing peptide (Cat. # PEP-026) is available for use in neutralization and control experiments.

Target Information

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Smad3 Mediates Diabetic Dyslipidemia and Fatty Liver in db/db Mice by Targeting PPARδ.

International journal of molecular sciences

He H,Zhong Y,Wang H,Tang PM,Xue VW,Chen X,Chen J,Huang X,Wang C,Lan H

Published figure using PPAR delta polyclonal antibody (Product # PA1-823A) in Western Blot

Thu Jul 13 00:00:00 UTC 2023

Low-Level Photobiomodulation Therapy Modulates H₂O₂ Production, TRPC-6, and PGC-1α Levels in the Dystrophic Muscle.

Photobiomodulation, photomedicine, and laser surgery

Covatti C,Mizobuti DS,Rocha GLD,da Silva HNM,de Lourenço CC,Pertille A,Pereira ECL,Minatel E

Published figure using PPAR delta polyclonal antibody (Product # PA1-823A) in Western Blot

Tue Aug 01 00:00:00 UTC 2023

Impaired PPARγ activation by cadmium exacerbates infection-induced lung injury.

JCI insight

Larson-Casey JL,Liu S,Pyles JM,Lapi SE,Saleem K,Antony VB,Gonzalez ML,Crossman DK,Carter AB

PA1-823A was used in Western Blot to suggest that impaired activation of PPARγ in monocyte-derived macrophages exacerbates lung injury and the severity of LRTIs.

Mon May 08 00:00:00 UTC 2023

Antioxidant effects of bis-indole alkaloid indigo and related signaling pathways in the experimental model of Duchenne muscular dystrophy.

Cell stress & chaperones

Mizobuti DS,da Rocha GL,da Silva HNM,Covatti C,de Lourenço CC,Pereira ECL,Salvador MJ,Minatel E

PA1-823A was used in Western Blotting to evaluate the potential role of the indigo on oxidative stress and related signaling pathways in primary skeletal muscle cell cultures and in the diaphragm muscle from mdx mice.

Fri Jul 01 00:00:00 UTC 2022

Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice.

Frontiers in physiology

da Silva HNM,Covatti C,da Rocha GL,Mizobuti DS,Mâncio RD,Hermes TA,Kido LA,Cagnon VHA,Pereira ECL,Minatel E

Published figure using PPAR delta polyclonal antibody (Product # PA1-823A) in Western Blot

Fri May 14 00:00:00 UTC 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-823A was used in Immunohistochemistry, Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 UTC 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-823A was used in Immunohistochemistry, Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 UTC 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-823A was used in Immunohistochemistry, Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 UTC 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-823A was used in Immunohistochemistry, Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 UTC 2021

β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.

Biomedicines

Hidalgo-Gutiérrez A,Barriocanal-Casado E,Díaz-Casado ME,González-García P,Zenezini Chiozzi R,Acuña-Castroviejo D,López LC

Published figure using PPAR delta polyclonal antibody (Product # PA1-823A) in Western Blot

Wed Oct 13 00:00:00 UTC 2021

ACOX2 is a prognostic marker and impedes the progression of hepatocellular carcinoma via PPARα pathway.

Cell death & disease

Zhang Q,Zhang Y,Sun S,Wang K,Qian J,Cui Z,Tao T,Zhou J

Published figure using PPAR delta polyclonal antibody (Product # PA1-823A) in Western Blot

Mon Jan 04 00:00:00 UTC 2021

Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors?

Protoplasma

Kotula-Balak M,Gorowska-Wojtowicz E,Milon A,Pawlicki P,Tworzydlo W,Płachno BJ,Krakowska I,Hejmej A,Wolski JK,Bilinska B

PA1-823A was used in Western Blot, Immunohistochemistry to investigate the G protein-coupled estrogen receptor (GPER) and peroxisome proliferator-activated receptor (PPAR) implication in regulation of lipid homeostasis including the expression of steroidogenesis-controlling molecules in clinical specimens of LCTs and tumor Leydig cells (MA-10).

Wed Jul 01 00:00:00 UTC 2020

Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors?

Protoplasma

Kotula-Balak M,Gorowska-Wojtowicz E,Milon A,Pawlicki P,Tworzydlo W,Płachno BJ,Krakowska I,Hejmej A,Wolski JK,Bilinska B

Wed Jul 01 00:00:00 UTC 2020

Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors?

Protoplasma

Kotula-Balak M,Gorowska-Wojtowicz E,Milon A,Pawlicki P,Tworzydlo W,Płachno BJ,Krakowska I,Hejmej A,Wolski JK,Bilinska B

Wed Jul 01 00:00:00 UTC 2020

Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation.

Cell communication and signaling : CCS

Hao YH,Lafita-Navarro MC,Zacharias L,Borenstein-Auerbach N,Kim M,Barnes S,Kim J,Shay J,DeBerardinis RJ,Conacci-Sorrell M

PA1-823A was used in Western Blotting to show myc regulates proliferation through activation of LEF1 expression and the WNT patway and that it is a transcriptional regulator of LEF1 in colonic cells.

Thu Oct 17 00:00:00 UTC 2019

Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation.

Cell communication and signaling : CCS

Hao YH,Lafita-Navarro MC,Zacharias L,Borenstein-Auerbach N,Kim M,Barnes S,Kim J,Shay J,DeBerardinis RJ,Conacci-Sorrell M

PA1-823A was used in Western Blotting to show myc regulates proliferation through activation of LEF1 expression and the WNT patway and that it is a transcriptional regulator of LEF1 in colonic cells.

Thu Oct 17 00:00:00 UTC 2019

Fatty Acid Synthesis and Degradation Interplay to Regulate the Oxidative Stress in Cancer Cells.

International journal of molecular sciences

Mikalayeva V,Ceslevičienė I,Sarapinienė I,Žvikas V,Skeberdis VA,Jakštas V,Bordel S

PA1-823A was used in Western Blotting to study whether fatty acid synthesis and fatty acid oxidation coexist in breast cancer cells.

Mon Mar 18 00:00:00 UTC 2019

Non-proteolytic ubiquitin modification of PPARγ by Smurf1 protects the liver from steatosis.

PLoS biology

Zhu K,Tang Y,Xu X,Dang H,Tang LY,Wang X,Wang XW,Zhang YE

PA1-823A was used in Western blotting following immunoprecipitation to demonstrate that mice deficient for ubiquitin ligase Smad ubiquitin regulatory factor 1 spontaneously develop hepatic steatosis as they age and exhibit the exacerbated phenotype under a high-fat diet.

Sat Dec 01 00:00:00 UTC 2018

Fasting Imparts a Switch to Alternative Daily Pathways in Liver and Muscle.

Cell reports

Kinouchi K,Magnan C,Ceglia N,Liu Y,Cervantes M,Pastore N,Huynh T,Ballabio A,Baldi P,Masri S,Sassone-Corsi P

PA1-823A was used in Western Blotting to study the effect of fasting on the circadian clock function.

Tue Dec 18 00:00:00 UTC 2018

PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.

Nature medicine

Dickey AS,Pineda VV,Tsunemi T,Liu PP,Miranda HC,Gilmore-Hall SK,Lomas N,Sampat KR,Buttgereit A,Torres MJ,Flores AL,Arreola M,Arbez N,Akimov SS,Gaasterland T,Lazarowski ER,Ross CA,Yeo GW,Sopher BL,Magnuson GK,Pinkerton AB,Masliah E,La Spada AR

PA1-823A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets

Fri Jan 01 00:00:00 UTC 2016

PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.

Nature medicine

PA1-823A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets

Fri Jan 01 00:00:00 UTC 2016

PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.

Nature medicine

PA1-823A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets

Fri Jan 01 00:00:00 UTC 2016

PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.

Nature medicine

PA1-823A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets

Fri Jan 01 00:00:00 UTC 2016

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.

Nature

Beyaz S,Mana MD,Roper J,Kedrin D,Saadatpour A,Hong SJ,Bauer-Rowe KE,Xifaras ME,Akkad A,Arias E,Pinello L,Katz Y,Shinagare S,Abu-Remaileh M,Mihaylova MM,Lamming DW,Dogum R,Guo G,Bell GW,Selig M,Nielsen GP,Gupta N,Ferrone CR,Deshpande V,Yuan GC,Orkin SH,Sabatini DM,Yilmaz ÖH

PA1-823A was used in western blot to characterize the enhancement of tumorigenicity and stemness of intestinal progenitors due to a high-fat diet

Thu Mar 03 00:00:00 UTC 2016

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.

Nature

PA1-823A was used in western blot to characterize the enhancement of tumorigenicity and stemness of intestinal progenitors due to a high-fat diet

Thu Mar 03 00:00:00 UTC 2016

Prenatal fat exposure and hypothalamic PPAR β/δ: Possible relationship to increased neurogenesis of orexigenic peptide neurons.

Peptides

Chang GQ,Karatayev O,Lukatskaya O,Leibowitz SF

PA1-823A was used in immunohistochemistry - frozen section to study the relationship between increased neurogenesis of orexigenic peptide neurons due to prenatal fat exposure and hypothalamic PPAR beta/delta

Sun May 01 00:00:00 UTC 2016

MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ-dependent manner.

Biochemical and biophysical research communications

Gong K,Qu B,Liao D,Liu D,Wang C,Zhou J,Pan X

PA1-823A was used in western blot to elucidate mechanisms by which miR-132 regulates osteogenic differentiation

Fri Sep 09 00:00:00 UTC 2016

Cardiomyocyte lipotoxicity is mediated by Il-6 and causes down-regulation of PPARs.

Biochemical and biophysical research communications

Haffar T,Bérubé-Simard FA,Bousette N

PA1-823A was used in western blot to study the effect of palmitate on cytokine and PPAR activity/expression in primary rat neonatal cardiomyocytes

Fri Mar 27 00:00:00 UTC 2015

Differentiation of epidermal keratinocytes is dependent on glucosylceramide:ceramide processing.

Human molecular genetics

Amen N,Mathow D,Rabionet M,Sandhoff R,Langbein L,Gretz N,Jäckel C,Gröne HJ,Jennemann R

PA1-823A was used in Western Blot to show that that Ugcg deletion led to deficiency of epidermal GlcCers and foremost to an increase of free extractable Cers and ωh-SMs. Sequential Cer-processing was blocked, indicated by drastic reduction of protein-bound Cers.

Tue Oct 15 00:00:00 UTC 2013

Skeletal muscle respiratory capacity is enhanced in rats consuming an obesogenic Western diet.

American journal of physiology. Endocrinology and metabolism

Stephenson EJ,Camera DM,Jenkins TA,Kosari S,Lee JS,Hawley JA,Stepto NK

PA1-823A was used in Western Blotting to evaluate the effects of Western diet-induced obesity on skeletal muscle mitochondrial function.

Fri Jun 15 00:00:00 UTC 2012

Inactivation of nuclear Wnt-beta-catenin signaling limits blastocyst competency for implantation.

Development (Cambridge, England)

Xie H,Tranguch S,Jia X,Zhang H,Das SK,Dey SK,Kuo CJ,Wang H

PA1-823A was used in immunohistochemistry to investigate the role of Wnt signaling for blastocyst competency for implantation

Fri Feb 01 00:00:00 UTC 2008

Neuregulins increase mitochondrial oxidative capacity and insulin sensitivity in skeletal muscle cells.

Diabetes

Cantó C,Pich S,Paz JC,Sanches R,Martínez V,Orpinell M,Palacín M,Zorzano A,Gumà A

PA1-823A was used in western blot to indicate the role of neuregulins in oxidative capacity and insulin sensitivity in muscle cells

Sat Sep 01 00:00:00 UTC 2007

Urine acidification has no effect on peroxisome proliferator-activated receptor (PPAR) signaling or epidermal growth factor (EGF) expression in rat urinary bladder urothelium.

Toxicology and applied pharmacology

Achanzar WE,Moyer CF,Marthaler LT,Gullo R,Chen SJ,French MH,Watson LM,Rhodes JW,Kozlosky JC,White MR,Foster WR,Burgun JJ,Car BD,Cosma GN,Dominick MA

PA1-823A was used in immunohistochemistry to investigate the influence of urine acidification on signal pathways in urinary bladder urothelium

Sat Sep 15 00:00:00 UTC 2007

Increase of peroxisome proliferator-activated receptor delta gene expression in the lungs of streptozotocin-induced diabetic rats.

Pulmonary pharmacology & therapeutics

Huang CJ,Liu IM,Cheng JT

PA1-823A was used in western blot to investigate PPAR delta expression in the lungs of diabetic rats induced by streptozotocin

Tue May 22 00:00:00 UTC 2007

Genetic- or transforming growth factor-beta 1-induced changes in epidermal peroxisome proliferator-activated receptor beta/delta expression dictate wound repair kinetics.

The Journal of biological chemistry

Tan NS,Michalik L,Desvergne B,Wahli W

PA1-823A was used in immunohistochemistry to demonstrate th effect of epidermal peroxisome proliferator-activated receptor beta/delta on wound repair kinetics.

Fri May 06 00:00:00 UTC 2005

PPAR gamma activators induce growth arrest and process extension in B12 oligodendrocyte-like cells and terminal differentiation of cultured oligodendrocytes.

Journal of neuroscience research

Roth AD,Leisewitz AV,Jung JE,Cassina P,Barbeito L,Inestrosa NC,Bronfman M

PA1-823A was used in western blot to investigate the effect of PPARgamma activation on the growth and morphology of B12 oligodendrocyte-like cells

Thu May 15 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: MGC3931; NUC1; NUCI; Nuclear hormone receptor 1; Nuclear receptor subfamily 1 group C member 2; OTTHUMP00000016256; OTTHUMP00000016257; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor delta; peroxisome proliferator activator receptor beta; peroxisome proliferator activator receptor, delta; peroxisome proliferator-activated nuclear receptor beta/delta variant 2; Peroxisome proliferator-activated receptor beta; Peroxisome proliferator-activated receptor delta; PPAR-beta; PPAR-delta; PPARdelta/beta

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Gene Aliases: FAAR; NR1C2; NUC-1; NUC1; NUCI; NUCII; PPAR-beta; PPAR-delta; PPAR[b]; PPARB; Pparb/d; PPARD; PPARdelta

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UniProt ID: (Human) Q03181, (Mouse) P35396

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Entrez Gene ID: (Human) 5467, (Rat) 25682, (Mouse) 19015

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Function(s)

transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding drug binding zinc ion binding lipid binding sequence-specific DNA binding protein heterodimerization activity NF-kappaB binding linoleic acid binding fatty acid binding enzyme activator activity prostacyclin receptor activity metal ion binding retinoid X receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PPAR delta Polyclonal Antibody

PPAR delta Antibody (PA1-823A) in ICC/IF

Product Details

PA1-823A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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