Invitrogen

PPAR gamma Polyclonal Antibody

PPAR gamma Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 28

PPAR gamma Antibody (PA3-821A) in ICC/IF

Immunofluorescence analysis of PPAR Gamma was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PPAR gamma Rabbit Polyclonal Antibody (Product # PA3-821A) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at r... View More

PPAR gamma Antibody in Western Blot (WB)

PPAR gamma Antibody in Immunohistochemistry (IHC)

PPAR gamma Antibody in Flow Cytometry (Flow)

PPAR gamma Antibody in Immunoprecipitation, ChIP Assay (IP, ChIP)

PPAR gamma Antibody in ChIP Assay (ChIP)

PPAR gamma Antibody in Mass Spectrometry (MS)

Product Image Gallery

PPAR gamma Antibody (PA3-821A) in — Figure 1 Nuclear binding assays. A , a partial SetD7 mouse promoter sequence curated using NM_080,793 as DNA reference sequence. The SetD7 PPRE sequence is bolded and underlined . The DNA sequences used for the primers designed for the ChIP assay PCR are bolded and italicized . B , consensus PPRE sequence on the SetD7 promoter: A putative PPARgamma response element was found using MatInspector software on the SetD7 promoter region that was conserved between mouse, rat, and human SetD7 promoter sequences. C , EMSA and super-shift assay of SetD7 PPRE: PAGE purified infrared dye (IRD-700) tagged oligos for SetD7 PPRE were annealed and EMSA was performed using nuclear extracts prepared from INS-1 cells as detailed in the Experimental procedures section. For the super-shift assay, INS-1 cell nuclear extracts were preincubated with a PPARgamma specific antibody or mouse nonimmune serum on ice for 30 min before adding infrared dye (IRD-700) tagged oligos for SetD7 PPRE. D , upper panel , ChIP assay PCR showing 144 base pairs (bp) SetD7 PPRE positive band: 300-400 bp chromatin preparations of betaTC6 cells were prepared as described in the Experimental procedures section. The prepared chromatin samples were immunoprecipitated using anti-PPARgamma versus a control nonimmune IgG, followed by PCR of the immunoprecipitated and nonimmunoprecipitated DNA (input DNA) using flanking primer pairs to the mouse SetD7-PPRE. The shown representative bands are the 144 bp (expected length) PCR prod

Product Details

PA3-821A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,500

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 3 publications 3 publications

Flow Cytometry (Flow)

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 3 publications 3 publications

Gel Shift (GS)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Mass Spectrometry (MS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(284) M G E D K I K F K H I T P L(298) of mouse PPAR gamma 2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2166056

Product Specific Information

PA3-821A detects peroxisome proliferator activated receptor (PPAR) gamma from human and mouse samples. This antibody is a reproduction of PA3-821, using the same immunizing peptide and new host rabbits.

PA3-821A has been successfully used in IF and Western blot procedures. Previous batches of this antibody have been used in gel shift, immunocytochemistry, and immunofluoresence procedures. By Western blot, this antibody detects a 57 kDa protein representing PPAR gamma 2 from differentiated mouse 3T3-L1 cell lysate. Immunolocalization of previous batches gives predominant nuclear staining.

The PA3-821A immunizing peptide corresponds to amino acid residues 284-298 from mouse PPAR gamma 2. This sequence is completely conserved in PPAR gamma 1, but exhibits no significant homology with PPAR alpha or NUC1. This sequence is completely conserved in mouse, human, and rat.

Target Information

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ZIKV Strains Elicit Different Inflammatory and Anti-Viral Responses in Microglia Cells.

Viruses

Oliveira FBC,Freire VPASS,Coelho SVA,Meuren LM,Palmeira JDF,Cardoso AL,Neves FAR,Ribeiro BM,Argañaraz GA,Arruda LB,Argañaraz ER

PA3-821A was used in Western Blot to conduct a comparative analysis of miRNAs (miRNA-155/146a/124) and their cellular targets (SOCS1/3, SHP1, TRAF6, IRAK1), as well as pro- and anti-inflammatory and anti-viral cytokines (IL-6, TNF-α, IFN-γ, IL-10, and IFN-β) and peroxisome proliferator-activated receptor γ (PPAR-γ) expression in BV2 microglia cells infected with ZIKV strains derived from African and Asian lineages (ZIKVMR766 and ZIKVPE243).

Fri May 26 00:00:00 UTC 2023

ZIKV Strains Elicit Different Inflammatory and Anti-Viral Responses in Microglia Cells.

Viruses

Oliveira FBC,Freire VPASS,Coelho SVA,Meuren LM,Palmeira JDF,Cardoso AL,Neves FAR,Ribeiro BM,Argañaraz GA,Arruda LB,Argañaraz ER

Fri May 26 00:00:00 UTC 2023

Tuberculosis alters immune-metabolic pathways resulting in perturbed IL-1 responses.

Frontiers in immunology

Llibre A,Smith N,Rouilly V,Musvosvi M,Nemes E,Posseme C,Mabwe S,Charbit B,Mbandi SK,Filander E,Africa H,Saint-André V,Bondet V,Bost P,Mulenga H,Bilek N,Albert ML,Scriba TJ,Duffy D

PA3-821A was used in Flow Cytometry to identify a novel immune-metabolic association between pregnane steroids, the PPARγ pathway and elevated plasma IL-1ra in TB.

Thu Jan 12 00:00:00 UTC 2023

Treatment with Amniotic Fluid Stem Cell Extracellular Vesicles Promotes Fetal Lung Branching and Cell Differentiation at Canalicular and Saccular Stages in Experimental Pulmonary Hypoplasia Secondary to Congenital Diaphragmatic Hernia.

Stem cells translational medicine

Khalaj K,Figueira RL,Antounians L,Gandhi S,Wales M,Montalva L,Biouss G,Zani A

PA3-821A was used in Immunohistochemistry (Paraffin) to suggest that AFSC-EV-based therapies hold potential for restoring fetal lung growth and maturation in babies with pulmonary hypoplasia secondary to CDH.

Fri Oct 21 00:00:00 UTC 2022

Stem cells translational medicine

Khalaj K,Figueira RL,Antounians L,Gandhi S,Wales M,Montalva L,Biouss G,Zani A

Fri Oct 21 00:00:00 UTC 2022

Breast cancer-associated skeletal muscle mitochondrial dysfunction and lipid accumulation is reversed by PPARG.

American journal of physiology. Cell physiology

Wilson HE,Stanton DA,Rellick S,Geldenhuys W,Pistilli EE

PA3-821A was used in Western Blotting to suggest that BC-derived factors alter lipid accumulation and mitochondrial function via different mechanisms that are both related to PPARG signaling, with mitochondrial dysfunction likely being altered via repression of PPAR-mediated transcription, and lipid accumulation being altered via transcription-independent functions of PPARG.

Thu Apr 01 00:00:00 UTC 2021

Decreased Substrate Stiffness Promotes a Hypofibrotic Phenotype in Cardiac Fibroblasts.

International journal of molecular sciences

Childers RC,Lucchesi PA,Gooch KJ

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in Immunocytochemistry

Wed Jun 09 00:00:00 UTC 2021

SetD7 (Set7/9) is a novel target of PPARγ that promotes the adaptive pancreatic β-cell glycemic response.

The Journal of biological chemistry

Jetton TL,Flores-Bringas P,Leahy JL,Gupta D

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in Protein Assays and Analysis

Mon Nov 01 00:00:00 UTC 2021

SetD7 (Set7/9) is a novel target of PPARγ that promotes the adaptive pancreatic β-cell glycemic response.

The Journal of biological chemistry

Jetton TL,Flores-Bringas P,Leahy JL,Gupta D

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in Protein Assays and Analysis

Mon Nov 01 00:00:00 UTC 2021

Cheonggukjang-Specific Component 1,3-Diphenyl-2-Propanone as a Novel PPARα/γ Dual Agonist: An In Vitro and In Silico Study.

International journal of molecular sciences

Arulkumar R,Jung HJ,Noh SG,Park D,Chung HY

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in Western Blot

Fri Oct 08 00:00:00 UTC 2021

Effects of Pioglitazone on Glucose-Dependent Insulinotropic Polypeptide-Mediated Insulin Secretion and Adipocyte Receptor Expression in Patients With Type 2 Diabetes.

Diabetes

Tharp WG,Gupta D,Sideleva O,Deacon CF,Holst JJ,Elahi D,Pratley RE

PA3-821A was used in Chromatin immunoprecipitation to show PPARγ agonists regulate GIP-R expression through PPREs in human adipocytes, but suggest this mechanism is not important for regulation of the insulinotropic effect of GIP in subjects with T2DM.

Sat Feb 01 00:00:00 UTC 2020

Human Breast Cancer Xenograft Model Implicates Peroxisome Proliferator-activated Receptor Signaling as Driver of Cancer-induced Muscle Fatigue.

Clinical cancer research : an official journal of the American Association for Cancer Research

Wilson HE,Rhodes KK,Rodriguez D,Chahal I,Stanton DA,Bohlen J,Davis M,Infante AM,Hazard-Jenkins H,Klinke DJ,Pugacheva EN,Pistilli EE

PA3-821A was used in Western Blotting to investigate if the patient-derieved orthotopic xenograft (PDOX) model would emulate the breast cancer-induced skeletal muscle fatigue seen commonly in patients with the absence of muscle wasting.

Mon Apr 01 00:00:00 UTC 2019

Rosiglitazone augments antioxidant response in the human trophoblast and prevents apoptosis†.

Biology of reproduction

Kohan-Ghadr HR,Kilburn BA,Kadam L,Johnson E,Kolb BL,Rodriguez-Kovacs J,Hertz M,Armant DR,Drewlo S

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in ChIP assay

Fri Feb 01 00:00:00 UTC 2019

Rosiglitazone augments antioxidant response in the human trophoblast and prevents apoptosis†.

Biology of reproduction

Kohan-Ghadr HR,Kilburn BA,Kadam L,Johnson E,Kolb BL,Rodriguez-Kovacs J,Hertz M,Armant DR,Drewlo S

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in ChIP assay

Fri Feb 01 00:00:00 UTC 2019

Peroxisome Proliferator-Activated Receptors (PPARs) levels in spermatozoa of normozoospermic and asthenozoospermic men.

Systems biology in reproductive medicine

Mousavi MS,Shahverdi A,Drevet J,Akbarinejad V,Esmaeili V,Sayahpour FA,Topraggaleh TR,Rahimizadeh P,Alizadeh A

PA3-821A was used in Flow cytometry/Cell sorting to propose a link between PPARγ gene expression level and motility in human sperm.

Sun Dec 01 00:00:00 UTC 2019

Peroxisome Proliferator-Activated Receptors (PPARs) levels in spermatozoa of normozoospermic and asthenozoospermic men.

Systems biology in reproductive medicine

Mousavi MS,Shahverdi A,Drevet J,Akbarinejad V,Esmaeili V,Sayahpour FA,Topraggaleh TR,Rahimizadeh P,Alizadeh A

PA3-821A was used in Flow cytometry/Cell sorting to propose a link between PPARγ gene expression level and motility in human sperm.

Sun Dec 01 00:00:00 UTC 2019

PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).

The ocular surface

Kim SW,Xie Y,Nguyen PQ,Bui VT,Huynh K,Kang JS,Brown DJ,Jester JV

PA3-821A was used in Western Blotting to evaluate the role of PPARγ in regulating meibocyte differentiation and lipid synthesis in a human meibomian gland epithelial cell line.

Mon Oct 01 00:00:00 UTC 2018

PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).

The ocular surface

Kim SW,Xie Y,Nguyen PQ,Bui VT,Huynh K,Kang JS,Brown DJ,Jester JV

PA3-821A was used in Western Blotting to evaluate the role of PPARγ in regulating meibocyte differentiation and lipid synthesis in a human meibomian gland epithelial cell line.

Mon Oct 01 00:00:00 UTC 2018

Duration of simulated microgravity affects the differentiation of mesenchymal stem cells.

Molecular medicine reports

Xue L,Li Y,Chen J

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in Flow Cytometry

Mon May 01 00:00:00 UTC 2017

PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically.

Nature medicine

Dickey AS,Pineda VV,Tsunemi T,Liu PP,Miranda HC,Gilmore-Hall SK,Lomas N,Sampat KR,Buttgereit A,Torres MJ,Flores AL,Arreola M,Arbez N,Akimov SS,Gaasterland T,Lazarowski ER,Ross CA,Yeo GW,Sopher BL,Magnuson GK,Pinkerton AB,Masliah E,La Spada AR

PA3-821A was used in immunohistochemistry and western blot to determine the role of decreased PPAR-delta in Huntington's disease and therapeutic targets

Fri Jan 01 00:00:00 UTC 2016

Heterogeneous Differentiation of Human Mesenchymal Stem Cells in 3D Extracellular Matrix Composites.

BioResearch open access

Jung JP,Bache-Wiig MK,Provenzano PP,Ogle BM

PA3-821A was used in immunocytochemistry to analyze 3D extracellular matrix composites for heterogeneous differentiation of human mesenchymal stem cells

Wed Feb 10 00:00:00 UTC 2016

Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.

The Journal of biological chemistry

Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA

PA3-821A was used in ChIP assay to investigate thyroid hormone receptor in adipogenesis

Fri Mar 20 00:00:00 UTC 2015

Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.

The Journal of biological chemistry

Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA

PA3-821A was used in ChIP assay to investigate thyroid hormone receptor in adipogenesis

Fri Mar 20 00:00:00 UTC 2015

Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.

The Journal of biological chemistry

Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA

PA3-821A was used in ChIP assay to investigate thyroid hormone receptor in adipogenesis

Fri Mar 20 00:00:00 UTC 2015

Pioglitazone-induced reductions in atherosclerosis occur via smooth muscle cell-specific interaction with PPAR{gamma}.

Circulation research

Subramanian V,Golledge J,Ijaz T,Bruemmer D,Daugherty A

Published figure using PPAR gamma polyclonal antibody (Product # PA3-821A) in Western Blot

Fri Oct 15 00:00:00 UTC 2010

Activation of PPAR-γ and PTEN cascade participates in lovastatin-mediated accelerated differentiation of oligodendrocyte progenitor cells.

Glia

Paintlia AS,Paintlia MK,Singh AK,Orak JK,Singh I

PA3-821A was used in western blot to investigate the mechanism for the differentiation of oligodendrocyte progenitor cells induced by lovastatin

Mon Nov 01 00:00:00 UTC 2010

Ligand activation of peroxisome proliferator-activated receptor-beta/delta and inhibition of cyclooxygenase-2 enhances inhibition of skin tumorigenesis.

Toxicological sciences : an official journal of the Society of Toxicology

Bility MT,Zhu B,Kang BH,Gonzalez FJ,Peters JM

PA3-821A was used in western blot to investigate therapeutic benefit of nonsteroidal anti-inflammatory drugs on skin tumorigenesis and its molecular mechanism

Fri Jan 01 00:00:00 UTC 2010

Effects of lysophosphatidic acid on the in vitro proliferation and differentiation of a novel porcine preadipocyte cell line.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Nobusue H,Kondo D,Yamamoto M,Kano K

PA3-821A was used in western blot to investigate the influence of lysophosphatidic acid on pig preadipocyte cell proliferation and differentiation

Wed Dec 01 00:00:00 UTC 2010

Urine acidification has no effect on peroxisome proliferator-activated receptor (PPAR) signaling or epidermal growth factor (EGF) expression in rat urinary bladder urothelium.

Toxicology and applied pharmacology

Achanzar WE,Moyer CF,Marthaler LT,Gullo R,Chen SJ,French MH,Watson LM,Rhodes JW,Kozlosky JC,White MR,Foster WR,Burgun JJ,Car BD,Cosma GN,Dominick MA

PA3-821A was used in immunohistochemistry to investigate the influence of urine acidification on signal pathways in urinary bladder urothelium

Sat Sep 15 00:00:00 UTC 2007

Peroxisomal proliferator-activated receptor-gamma agonists induce partial reversion of epithelial-mesenchymal transition in anaplastic thyroid cancer cells.

Endocrinology

Aiello A,Pandini G,Frasca F,Conte E,Murabito A,Sacco A,Genua M,Vigneri R,Belfiore A

PA3-821 was used in western blot to investigate the role of PPAR gamma in the tumorigenesis of anaplastic thyroid cancer cells.

Fri Sep 01 00:00:00 UTC 2006

Reciprocal expression of peroxisome proliferator-activated receptor-gamma and cyclooxygenase-2 in human term parturition.

American journal of obstetrics and gynecology

Dunn-Albanese LR,Ackerman WE,Xie Y,Iams JD,Kniss DA

PA3-821A was used in western blot to study the involvement of peroxisome proliferator-activated receptor-gamma and cyclooxygenase-2 in human term parturition

Mon Mar 01 00:00:00 UTC 2004

Dexamethasone induced preadipocyte recruitment and expression of CCAAT/enhancing binding protein alpha and peroxisome proliferator activated receptor-gamma proteins in porcine stromal-vascular (S-V) cell cultures obtained before and after the onset of fetal adipogenesis.

General and comparative endocrinology

Hausman GJ

PA3-821A was used in immunohistochemistry to investigate the effect of dexamethasone on preadipocyte recruitment and transcription factor expression in stromal-vascular cells

Fri Aug 01 00:00:00 UTC 2003

Enhanced growth inhibition by combination differentiation therapy with ligands of peroxisome proliferator-activated receptor-gamma and inhibitors of histone deacetylase in adenocarcinoma of the lung.

Clinical cancer research : an official journal of the American Association for Cancer Research

Chang TH,Szabo E

PA3-821 was used in western blot to investigate the role of the HDAC inhibitors in non-small cell lung cancer.

Mon Apr 01 00:00:00 UTC 2002

Human translocation liposarcoma-CCAAT/enhancer binding protein (C/EBP) homologous protein (TLS-CHOP) oncoprotein prevents adipocyte differentiation by directly interfering with C/EBPbeta function.

The Journal of biological chemistry

Adelmant G,Gilbert JD,Freytag SO

PA3-821 was used in immunocytochemistry to demonstrate that TLS-CHOP blocks adipocyte differentiation by directly interfering with C/EBPbeta function

Fri Jun 19 00:00:00 UTC 1998

MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response.

Molecular and cellular endocrinology

Kilgore MW,Tate PL,Rai S,Sengoku E,Price TM

PA3-821 was used in EMSA assay to characterize MCF-7 and T47D human breast cancer cells in terms of peroxisomal response

Fri May 16 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: HUMPPARG; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARy

View more View less

Gene Aliases: CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2

View more View less

UniProt ID: (Human) P37231, (Mouse) P37238, (Rat) O88275

View more View less

Entrez Gene ID: (Human) 5468, (Mouse) 19016, (Rat) 25664

View more View less

Function(s)

core promoter sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein phosphatase binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity activating transcription factor binding identical protein binding sequence-specific DNA binding transcription regulatory region DNA binding retinoid X receptor binding arachidonic acid binding alpha-actinin binding RNA polymerase II regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding metal ion binding WW domain binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PPAR gamma Polyclonal Antibody

PPAR gamma Antibody (PA3-821A) in ICC/IF

Product Details

PA3-821A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Miscellaneous PubMed (MISC)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!