Proteintech

PPARA Monoclonal Antibody (1G1E10)

1 Published Figure

29 References

View all (34) PPAR alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

FIGURE: 1 / 3

PPARA Antibody (66826-1-IG) in WB

HepG2 cells were subjected to SDS PAGE followed by western blot with 66826-1-IG (PPARA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Product Details

66826-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1G1E10

Immunogen

PPARA Fusion Protein Ag7896 (1-258 aa encoded by BC000052)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MVDTESPLC PLSPLEAGDL ESPLSEEFLQ EMGNIQEISQ SIGEDSSGSF GFTEYQYLGS CPGSDGSVIT DTLSPASSPS SVTYPVVPGS VDESPSGALN IECRICGDKA SGYHYGVHAC EGCKGFFRRT IRLKLVYDKC DRSCKIQKKN RNKCQYCRFH KCLSVGMSHN AIRFGRMPRS EKAKLKAEIL TCEHDIEDSE TADLKSLAKR IYEAYLKNFN MNKVKARVIL SGKASNNPVG VCGCSGFSWQ HGTSVVEDD (1-258 aa encoded by BC000052 )

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Muscular lipidomics and transcriptomics reveal the effects of bile acids on lipid metabolism in high-fat diet-fed grouper.

Fish physiology and biochemistry

Xu J,Shi M,Chen L,Chi S,Zhang S,Cao J,Tan B,Xie S

Thu Feb 01 00:00:00 UTC 2024

Hepatocyte-derived extracellular vesicles miR-122-5p promotes hepatic ischemia reperfusion injury by regulating Kupffer cell polarization.

International immunopharmacology

Liu L,Xiao F,Sun J,Wang Q,Wang A,Zhang F,Li Z,Wang X,Fang Z,Qiao Y

Thu Jun 01 00:00:00 UTC 2023

A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer.

Frontiers in pharmacology

Li SC,Yan LJ,Wei XL,Jia ZK,Yang JJ,Ning XH

Sun May 21 00:00:00 UTC 2023

Deep Sea Water Inhibited Pancreatic β-Cell Apoptosis and Regulated Glucose Homeostasis by Affecting Lipid Metabolism in Db/Db Mice.

Diabetes, metabolic syndrome and obesity : targets and therapy

Shi M,Hu B,Hong Y,Wang M,Yao Y

Sat Feb 11 00:00:00 UTC 2023

Supplemental Clostridium butyricum modulates lipid metabolism by reshaping the gut microbiota composition and bile acid profile in IUGR suckling piglets.

Journal of animal science and biotechnology

Zhang X,Yun Y,Lai Z,Ji S,Yu G,Xie Z,Zhang H,Zhong X,Wang T,Zhang L

Mon Mar 13 00:00:00 UTC 2023

Derivation and comprehensive analysis of ageing-related genes in intervertebral disc degeneration for prediction and immunology.

Mechanisms of ageing and development

Xu S,Fu H,Weng S,Gu X,Li J

Sat Apr 01 00:00:00 UTC 2023

Anti-inflammatory effect of Irisin on LPS-stimulated macrophages through inhibition of MAPK pathway.

Physiological research

Ma Y,Du Y,Yang J,He Q,Wang H,Lin X

Sun Apr 30 00:00:00 UTC 2023

Butyrate mitigates metabolic dysfunctions via the ERα-AMPK pathway in muscle in OVX mice with diet-induced obesity.

Cell communication and signaling : CCS

Fu Q,Li T,Zhang C,Ma X,Meng L,Liu L,Shao K,Wu G,Zhu X,Zhao X

Thu May 04 00:00:00 UTC 2023

Insoluble Dietary Fiber from Soybean Residue (Okara) Exerts Anti-Obesity Effects by Promoting Hepatic Mitochondrial Fatty Acid Oxidation.

Foods (Basel, Switzerland)

Zhang J,Wang S,Wang J,Liu W,Gong H,Zhang Z,Lyu B,Yu H

Mon May 22 00:00:00 UTC 2023

Diosgenin attenuates nonalcoholic hepatic steatosis through the hepatic FXR-SHP-SREBP1C/PPARα/CD36 pathway.

European journal of pharmacology

Chen S,Sun S,Feng Y,Li X,Yin G,Liang P,Yu W,Meng D,Zhang X,Liu H,Zhang F

Sat Aug 05 00:00:00 UTC 2023

Theabrownin of raw and ripened pu-erh tea varies in the alleviation of HFD-induced obesity via the regulation of gut microbiota.

European journal of nutrition

Deng X,Zhang N,Wang Q,Huang Y,Huang Y,Lin Y,Huang M,Zheng F,Xiao M,Ye J

Tue Aug 01 00:00:00 UTC 2023

Modulation of the Microbiome-Fat-Liver Axis by Lactic Acid Bacteria: A Potential Alleviated Role in High-Fat-Diet-Induced Obese Mice.

Journal of agricultural and food chemistry

Wei B,Peng Z,Xiao M,Huang T,Yang S,Liu K,Wu M,Zheng W,Xie M,Xiong T

Wed Jul 12 00:00:00 UTC 2023

Local glucose elevation activates pyroptosis via NLRP3 inflammasome in ovarian granulosa cells of overweight patients.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Xu R,Zhao H,Qi J,Yao G,He Y,Lu Y,Zhu Q,Wang Y,Ding Y,Zhu Z,Li X,Vankelecom H,Sun Y

Wed Mar 01 00:00:00 UTC 2023

Bifidobacterium animalis subsp. lactis A6 Enhances Fatty Acid β-Oxidation of Adipose Tissue to Ameliorate the Development of Obesity in Mice.

Nutrients

Huo Y,Zhao G,Li J,Wang R,Ren F,Li Y,Wang X

Sat Jan 29 00:00:00 UTC 2022

Effects of heat-processed Gynostemma pentaphyllum on high-fat diet-fed mice of obesity and functional analysis on network pharmacology and molecular docking strategy.

Journal of ethnopharmacology

Xie P,Guo M,Xie JB,Xiao MY,Qi YS,Duan Y,Li FF,Piao XL

Wed Aug 10 00:00:00 UTC 2022

RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis.

Cell death and differentiation

Yin X,Teng X,Ma T,Yang T,Zhang J,Huo M,Liu W,Yang Y,Yuan B,Yu H,Huang W,Wang Y

Tue Nov 01 00:00:00 UTC 2022

RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis.

Cell death and differentiation

Yin X,Teng X,Ma T,Yang T,Zhang J,Huo M,Liu W,Yang Y,Yuan B,Yu H,Huang W,Wang Y

Tue Nov 01 00:00:00 UTC 2022

Farnesoid X receptor regulates PI₃K/AKT/mTOR signaling pathway, lipid metabolism, and immune response in hybrid grouper.

Fish physiology and biochemistry

Xu J,Yao X,Li X,Xie S,Chi S,Zhang S,Cao J,Tan B

Thu Dec 01 00:00:00 UTC 2022

Ban-xia-xie-xin-tang ameliorates hepatic steatosis by regulating Cidea and Cidec expression in HFD-fed mice.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xia QS,Gao Y,Wen-Bin W,Wu F,Dong H,Xu LJ,Fang K,Hu ML,Yuan F,Lu FE,Gong J

Sat Oct 01 00:00:00 UTC 2022

Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease.

Nature communications

Lekka E,Kokanovic A,Mosole S,Civenni G,Schmidli S,Laski A,Ghidini A,Iyer P,Berk C,Behera A,Catapano CV,Hall J

Mon Dec 26 00:00:00 UTC 2022

Pterostilbene and Its Derivative 3'-Hydroxypterostilbene Ameliorated Nonalcoholic Fatty Liver Disease Through Synergistic Modulation of the Gut Microbiota and SIRT1/AMPK Signaling Pathway.

Journal of agricultural and food chemistry

Tsai HY,Shih YY,Yeh YT,Huang CH,Liao CA,Hu CY,Nagabhushanam K,Ho CT,Chen YK

Wed Apr 27 00:00:00 UTC 2022

Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota.

Frontiers in pharmacology

Yang S,Cao S,Li C,Zhang J,Liu C,Qiu F,Kang N

Tue Jul 26 00:00:00 UTC 2022

Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes.

International journal of molecular sciences

Li Z,Zhang Q,Liu X,Zhao M

Wed Nov 23 00:00:00 UTC 2022

Dynein light chain LC8 alleviates nonalcoholic steatohepatitis by inhibiting NF-κB signaling and reducing oxidative stress.

Journal of cellular physiology

Lee GR,Lee HI,Kim N,Lee J,Kwon M,Kang YH,Song HJ,Yeo CY,Jeong W

Thu Sep 01 00:00:00 UTC 2022

Loss of mitochondrial ATPase ATAD3A contributes to nonalcoholic fatty liver disease through accumulation of lipids and damaged mitochondria.

The Journal of biological chemistry

Chen L,Li Y,Sottas C,Lazaris A,Petrillo SK,Metrakos P,Li L,Ishida Y,Saito T,Garza S,Papadopoulos V

Wed Jun 01 00:00:00 UTC 2022

Probiotic-fermented rice buckwheat alleviates high-fat diet-induced hyperlipidemia in mice by suppressing lipid accumulation and modulating gut microbiota.

Food research international (Ottawa, Ont.)

Yan J,Xue Q,Chen W,Wang K,Peng D,Jiang J,Li P,Du B

Sun May 01 00:00:00 UTC 2022

Effect of fenofibrate on proliferation of SMMC-7721 cells via regulating cell cycle.

Human & experimental toxicology

Li B,Jiang HY,Wang ZH,Ma YC,Bao YN,Jin Y

Thu Jul 01 00:00:00 UTC 2021

Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.

Alcohol (Fayetteville, N.Y.)

Chen M,Sun X,Wei W,Cucarella C,Martín-Sanz P,Casado M,Pi L,Ren B,Cao Q

Sat May 01 00:00:00 UTC 2021

Effects of dibutyl phthalate on lipid metabolism in liver and hepatocytes based on PPARα/SREBP-1c/FAS/GPAT/AMPK signal pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Zhang W,Li JY,Wei XC,Wang Q,Yang JY,Hou H,Du ZW,Wu XA

Mon Mar 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha

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Gene Aliases: hPPAR; NR1C1; PPAR; PPARA; PPARalpha

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UniProt ID: (Human) Q07869, (Rat) P37230

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Entrez Gene ID: (Human) 5465, (Rat) 25747

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription coactivator binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding drug binding zinc ion binding lipid binding phosphatase binding protein domain specific binding ubiquitin conjugating enzyme binding protein complex binding sequence-specific DNA binding NFAT protein binding MDM2/MDM4 family protein binding receptor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PPARA Monoclonal Antibody (1G1E10)

PPARA Antibody (66826-1-IG) in WB

Product Details

66826-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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