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PPCS Antibody (MA5-36234) in IHC (P)
Immunohistochemistry analysis of PPCS in paraffin-embedded mouse cardiac muscle tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PPCS monoclonal antibody (Product # MA5-36234) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB... View More
Product Details
MA5-36234
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
7G13
Immunogen
A synthetic peptide corresponding to a sequence of human PPCS (VEIEEKIVDNLQSRHTAFIGDRN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884068
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A375 whole cell, human COLO-320 whole cell. IHC: human tonsil tissue, mouse cardiac muscle tissue, mouse cardiac muscle tissue, rat cardiac muscle tissue. Flow: PC-3 cell, SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Phosphopantothenate--cysteine ligase; Phosphopantothenoylcysteine synthetase; PPC synthetase
Gene Aliases: 6330579B17Rik; AI413471; AI790434; COAB; PPCS; RGD1311399
UniProt ID: (Human) Q9HAB8, (Mouse) Q8VDG5
Entrez Gene ID: (Human) 79717, (Mouse) 106564, (Rat) 298490
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