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Invitrogen
PPM1F Polyclonal Antibody
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PPM1F Antibody (PA5-116618) in WB
Western blot analysis of PPM1F in extracts of various cell lines. Samples were incubated with PPM1F Polyclonal antibody (Product # PA5-116618) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-116618
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PPM1F (NP_0554491)
Conjugate
Form
Liquid
Concentration
1.16 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901249
Product Specific Information
Positive Samples: MCF7, HeLa, HepG2, K-562
Immunogen sequence: MSSGAPQKSS PMASGAEETP GFLDTLLQDF PALLNPEDPL PWKAPGTVLS QEEVEGELAE LAMGFLGSRK APPPLAAALA HEAVSQLLQT DLSEFRKLPR EEEEEEEDDD EEEKAPVTLL DAQSLAQSFF NRLWEVAGQW QKQVPLAARA SQRQWLVSIH AIRNTRRKME DRHVSLPSFN QLFGLSDPVN
Target Information
PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; hFem-2; Partner of PIX 2; PP2C phosphatase; Protein fem-2 homolog; Protein phosphatase 1F; protein phosphatase 1F (PP2C domain containing); protein phosphatase, Mg2+/Mn2+ dependent, 1F
Gene Aliases: CAMKP; CaMKPase; FEM-2; hFEM-2; KIAA0015; POPX2; PPM1F
UniProt ID: (Human) P49593
Entrez Gene ID: (Human) 9647
negative regulation of protein kinase activity
positive regulation of gene expression
positive regulation of epithelial cell migration
positive regulation of cell-substrate adhesion
histone dephosphorylation
negative regulation of peptidyl-serine phosphorylation
cellular response to drug
peptidyl-threonine dephosphorylation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of protein kinase activity by regulation of protein phosphorylation
negative regulation of transcription, DNA-templated
positive regulation of growth
positive regulation of chemotaxis
positive regulation of stress fiber assembly
positive regulation of focal adhesion assembly
intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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