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Invitrogen
PPP1R16B Polyclonal Antibody
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FIGURE: 1 / 2
PPP1R16B Antibody (PA5-101782) in ICC/IF
Immunocytochemistry analysis of PPP1R16B in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PPP1R16B polyclonal antibody (Product # PA5-101782) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101782
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PPP1R16B(Accession Q96T49), corresponding to amino acid residues E363-T413.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2851214
Product Specific Information
Antibody detects endogenous levels of total PPP1R16B.
Target Information
The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ankyrin repeat domain protein 4; Ankyrin repeat domain-containing protein 4; CAAX box protein TIMAP; hTIMAP; Protein phosphatase 1 regulatory inhibitor subunit 16B; protein phosphatase 1, regulatory (inhibitor) subunit 16B; protein phosphatase 1, regulatory subunit 16B; TGF-beta-inhibited membrane-associated protein; whn-dependent transcript 4
Gene Aliases: ANKRD4; C130078N17Rik; KIAA0823; PPP1R16B; TIMAP; Wdt4
UniProt ID: (Human) Q96T49, (Mouse) Q8VHQ3
Entrez Gene ID: (Human) 26051, (Mouse) 228852
positive regulation of endothelial cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of protein dephosphorylation
negative regulation of protein dephosphorylation
regulation of catalytic activity
establishment of endothelial barrier
negative regulation of peptidyl-serine dephosphorylation
positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
regulation of filopodium assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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