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PPP2R1A Antibody (A300-962A) in IP
Detection of human PPP2R1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R1A antibody (Product # A300-962A) (lot 2) used for IP at 3 µg per reaction. PPP2R1A was also immunoprecipitated by rabbit anti-PPP2R1A antibody A300-963A. For blotting immunoprecipitated PPP2R1A, A300-962A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-962A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of Human protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Bovine and Pig
Target Information
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Medium tumor antigen-associated 61 kDa protein; MGC786; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2Aa; PP2Ac; PP2CA; PP2Calpha; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; protein phosphatase 2 regulatory subunit A, alpha; protein phosphatase 2, regulatory subunit A, alpha; RP-C; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; testicular secretory protein Li 1
Gene Aliases: MRD36; PP2A-Aalpha; PP2AAALPHA; PPP2R1A; PR65A
UniProt ID: (Human) P30153
Entrez Gene ID: (Human) 5518
G2/M transition of mitotic cell cycle
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
inactivation of MAPK activity
regulation of DNA replication
regulation of transcription, DNA-templated
protein complex assembly
protein dephosphorylation
ceramide metabolic process
apoptotic process
chromosome segregation
mitotic nuclear envelope reassembly
female meiotic division
RNA splicing
response to organic substance
second-messenger-mediated signaling
regulation of Wnt signaling pathway
regulation of cell adhesion
negative regulation of cell growth
regulation of protein phosphatase type 2A activity
regulation of growth
negative regulation of tyrosine phosphorylation of Stat3 protein
regulation of cell differentiation
meiotic spindle elongation
mitotic sister chromatid separation
meiotic sister chromatid cohesion, centromeric
peptidyl-serine dephosphorylation
regulation of meiotic cell cycle process involved in oocyte maturation
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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