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PPP2R5D Antibody (A301-099A) in IP
Detection of human PPP2R5D by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP2R5D antibody (Product # A301-099A) used for IP at 3 µg/mg lysate. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies A301-098A and A301-100A, which recognize other epitopes. For blotting immunoprecipitated PPP2R5D, A301-098A (A) and A301-100A (B) were used at 0.1 µg/mL. Detection: Chemiluminescence with exposure times of 10 seconds (A) ... View More
Product Details
A301-099A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 500 and 550 of Human serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rabbit
Target Information
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF265 (Zinc finger protein 265), also known as ZRANB2 (Zinc finger Ran-binding domain-containing protein 2), ZIS, ZIS1 or ZIS2, is a 330 amino acid protein that belongs to the ZRANB2 family. Localized to the nucleus, ZNF265 functions as a splicing factor that is responsible for alternatively splicing Tra-2 beta (transformer-2 beta) transcripts and is thought to interfere with constitutive 5'-splice selection. ZNF265 contains two RanBP2-type zinc fingers through which it conveys its RNA-binding activity. Two isoforms, designated ZIS-1 and ZIS-2, are expressed due to alternative splicing events. Upon DNA damage, ZIS-2 may be phosphorylated by ATM or ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC2134; MGC8949; PP2A B subunit isoform B'-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta; PP2A, B subunit, B' delta isoform; PP2A, B subunit, B56 delta isoform; PP2A, B subunit, PR61 delta isoform; PP2A, B subunit, R5 delta isoform; PP2CB; protein phosphatase 2, regulatory subunit B (B56), delta isoform; Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Gene Aliases: B56D; MRD35; PPP2R5D
UniProt ID: (Human) Q14738
Entrez Gene ID: (Human) 5528
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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