Proteintech

PR Polyclonal Antibody

PR Antibody in Immunocytochemistry (ICC/IF)

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PR Antibody (25871-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed T-47D cells using PR antibody (Product # 25871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

25871-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PR Fusion Protein Ag22986 (1-301 aa encoded by NM_000926)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTELKAKGP RAPHVAGGPP SPEVGSPLLC RPAAGPFPGS QTSDTLPEVS AIPISLDGLL FPRPCQGQDP SDEKTQDQQS LSDVEGAYSR AEATRGAGGS SSSPPEKDSG LLDSVLDTLL APSGPGQSQP SPPACEVTSS WCLFGPELPE DPPAAPATQR VLSPLMSRSG CKVGDSSGTA AAHKVLPRGL SPARQLLLPA SESPHWSGAP VKPSPQAAAV EVEEEDGSES EESAGPLLKG KPRALGGAAA GGGAAAVPPG AAAGGVALVP KEDSRFSAPR VALVEQDAPM APGRSPLATT VM (1-301 aa encoded by NM_000926)

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A NOTCH1 Mutation Found in a Newly Established Ovarian Cancer Cell Line (FDOVL) Promotes Lymph Node Metastasis in Ovarian Cancer.

International journal of molecular sciences

Jiang W,Ouyang X,Jiang C,Yin L,Yao Q,Pei X,Ji Z,Li M,Song S,Yang W,Huang S,Yang H,Shan B

Tue Mar 07 00:00:00 UTC 2023

The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats.

International journal of molecular sciences

Gao Z,Shao D,Zhao C,Liu H,Zhao X,Wei Q,Ma B

Tue May 16 00:00:00 UTC 2023

The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats.

International journal of molecular sciences

Gao Z,Shao D,Zhao C,Liu H,Zhao X,Wei Q,Ma B

Tue May 16 00:00:00 UTC 2023

Time-Restricted Feeding Ameliorates Uterine Epithelial Estrogen Receptor α Transcriptional Activity at the Time of Embryo Implantation in Mice Fed a High-Fat Diet.

The Journal of nutrition

Liu L,Zhuo Y,Zhang H,Li J,Jiang X,Han X,Chao J,Feng B,Che L,Xu S,Lin Y,Li J,Fang Z,Sun M,Luo T,Wu D,Hua L

Thu Jun 01 00:00:00 UTC 2023

Effect of acupuncture on the opening time of implantation window and endometrial receptivity in controlled ovarian hyperstimulation rats during peri-implantation period.

Frontiers in endocrinology

Hu R,Huang Y,Song Y,Wu X,Song K,Huang G,Zhang M,Dong H

Tue Apr 04 00:00:00 UTC 2023

Effect of acupuncture on the opening time of implantation window and endometrial receptivity in controlled ovarian hyperstimulation rats during peri-implantation period.

Frontiers in endocrinology

Hu R,Huang Y,Song Y,Wu X,Song K,Huang G,Zhang M,Dong H

Tue Apr 04 00:00:00 UTC 2023

SERD-NHC-Au(I) complexes for dual targeting ER and TrxR to induce ICD in breast cancer.

Pharmacological research

Lu Y,Sheng X,Liu C,Liang Z,Wang X,Liu L,Wen Z,Yang Z,Du Q,Liu W

Sat Apr 01 00:00:00 UTC 2023

Upregulation of estrogen receptor alpha (ERα) expression in transgenic mice expressing human CYP4Z1.

Breast cancer research and treatment

Khayeka-Wandabwa C,Zhao J,Pathak JL,Wu H,Bureik M

Sat Jan 01 00:00:00 UTC 2022

Hybrid Broussonetia papyrifera Fermented Feed Can Play a Role Through Flavonoid Extracts to Increase Milk Production and Milk Fatty Acid Synthesis in Dairy Goats.

Frontiers in veterinary science

Zhao M,Lv D,Hu J,He Y,Wang Z,Liu X,Ran B,Hu J

Sat Apr 02 00:00:00 UTC 2022

ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.

Nucleic acids research

Park J,Lee J,Kim JH,Lee J,Park H,Lim C

Mon Nov 08 00:00:00 UTC 2021

ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.

Nucleic acids research

Park J,Lee J,Kim JH,Lee J,Park H,Lim C

Mon Nov 08 00:00:00 UTC 2021

Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance.

Nature communications

Deng Y,Xia X,Zhao Y,Zhao Z,Martinez C,Yin W,Yao J,Hang Q,Wu W,Zhang J,Yu Y,Xia W,Yao F,Zhao D,Sun Y,Ying H,Hung MC,Ma L

Mon Dec 06 00:00:00 UTC 2021

Bushen Huoxue recipe attenuates early pregnancy loss via activating endometrial COX2-PGE2 angiogenic signaling in mice.

BMC complementary medicine and therapies

Song Y,Zhou F,Tan X,Liu X,Ding J,Zhang C,Li F,Zhu W,Ma W,Hu R,Zhang M

Thu Jan 14 00:00:00 UTC 2021

The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters.

Reproductive sciences (Thousand Oaks, Calif.)

Goto K,Kawano Y,Utsunomiya T,Narahara H

Wed Sep 01 00:00:00 UTC 2021

Pan-Cancer Prognostic Role and Targeting Potential of the Estrogen-Progesterone Axis.

Frontiers in oncology

Shen YT,Huang X,Zhang G,Jiang B,Li CJ,Wu ZS

Mon Apr 25 00:00:00 UTC 2022

Expression of hormone receptors predicts survival and platinum sensitivity of high-grade serous ovarian cancer.

Bioscience reports

Tan J,Song C,Wang D,Hu Y,Liu D,Ma D,Gao Q

Fri May 28 00:00:00 UTC 2021

BML-111 inhibits EMT, migration and metastasis of TAMs-stimulated triple-negative breast cancer cells via ILK pathway.

International immunopharmacology

Lin L,Luo X,Wang L,Xu F,He Y,Wang Q,Yuan C,Xu J,Yan L,Hao H

Sat Aug 01 00:00:00 UTC 2020

G-Protein-Coupled Estrogen Receptor 1 Promotes Gender Disparities in Hepatocellular Carcinoma via Modulation of SIN1 and mTOR Complex 2 Activity.

Molecular cancer research : MCR

Feng G,Cai J,Huang Y,Zhu X,Gong B,Yang Z,Yan C,Hu Z,Yang L,Wang Z

Tue Dec 01 00:00:00 UTC 2020

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Acta neuropathologica

Riku Y,Duyckaerts C,Boluda S,Plu I,Le Ber I,Millecamps S,Salachas F,Yoshida M,Ando T,Katsuno M,Sobue G,Seilhean D

Fri Nov 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor

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Gene Aliases: 9930019P03Rik; BB114106; ENSMUSG00000074510; NR3C3; PGR; PR; PR-A; PR-B

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UniProt ID: (Human) P06401, (Mouse) Q00175

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Entrez Gene ID: (Human) 5241, (Mouse) 18667

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity steroid binding protein binding zinc ion binding enzyme binding ATPase binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding lipid binding hormone binding sequence-specific DNA binding metal ion binding

Process(es)

transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling negative regulation of gene expression steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter ovulation from ovarian follicle epithelial cell maturation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of smooth muscle cell migration intracellular receptor signaling pathway mammary gland development paracrine signaling response to cocaine negative regulation of apoptotic process positive regulation of transcription, DNA-templated lung alveolus development regulation of epithelial cell proliferation progesterone receptor signaling pathway female mating behavior tertiary branching involved in mammary gland duct morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PR Polyclonal Antibody

PR Antibody (25871-1-AP) in ICC/IF

Product Details

25871-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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