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Invitrogen
PRC1 Polyclonal Antibody, HRP
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PRC1 Antibody (PA1-27244) in WB
PRC1 Polyclonal Antibody, HRP (Product # PA1-27244) was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1µg of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (Overnight at 4°C in blocking buffer). Detection shown was using Dylight 488 conjµgated secondary antibody (1:10K in TBS/blocking buffer 1 hr RT).
Product Details
PA1-27244
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Published species
Fungi
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carboxypeptidase Y (S. cerevisiae (Baker's Yeast)).
Conjugate
Form
Liquid
Concentration
10 mg/mL
Purification
IgG fraction
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.01% gentamicin sulfate
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_779510
Product Specific Information
PA1-27244 detects Carboxypeptidase Y from S. cerevisiae samples.
Target Information
This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: carboxypeptidase C PRC1; Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; MGC1671; MGC3669
Gene Aliases: LBC1; PRC1; YMR297W
UniProt ID: (Baker's yeast) P00729
Entrez Gene ID: (Baker's yeast) 855343
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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