Invitrogen

PRDM16 Polyclonal Antibody

19 Published Figures

17 References

View all (4) PRDM16 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (19)

PRDM16 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

PRDM16 Antibody (PA5-20872) in ICC/IF

Immunofluorescence of PRDM16 in Human Brain cells with PRDM16 Polyclonal Antibody (Product # PA5-20872) at 20 µg/mL.

PRDM16 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-20872

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 16 publications 16 publications

Immunohistochemistry (IHC)

2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

20 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 17 amino acid peptide from near the carboxy terminus of human PRDM16.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11154178

Product Specific Information

PA5-20872 can be used with blocking peptide PEP-0986.

Target Information

PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exercise improves homeostasis of the intestinal epithelium by activation of apelin receptor-AMP-activated protein kinase signalling.

The Journal of physiology

Chae SA,Du M,Son JS,Zhu MJ

PA5-20872 was used in Western Blot to demonstrate that exercise-induced activation of the APJ-AMPK axis facilitates the homeostasis of the intestinal duodenal epithelium.

Thu Jun 01 00:00:00 UTC 2023

Exercise improves homeostasis of the intestinal epithelium by activation of apelin receptor-AMP-activated protein kinase signalling.

The Journal of physiology

Chae SA,Du M,Son JS,Zhu MJ

PA5-20872 was used in Western Blot to demonstrate that exercise-induced activation of the APJ-AMPK axis facilitates the homeostasis of the intestinal duodenal epithelium.

Thu Jun 01 00:00:00 UTC 2023

Maternal secretin ameliorates obesity by promoting white adipose tissue browning in offspring.

EMBO reports

Xue L,Sun J,Liu J,Hu C,Wu D,Nie C,Zhang K,Wang Y,Zhao L,Li X,Lu Y,Zhang L,Zhang D,Fan M,Qian H,Jiang H,Wong J,Li Y,Ying H,Chow BK,Wang L,Li Y

PA5-20872 was used in Western Blotting to show that SCT treatment during pregnancy can reduce the development of obesity and improve glucose tolerance and insulin resistance in offspring of HFD-fed females, suggesting that SCT may serve as a novel biomarker or a strategy for preventing metabolic diseases.

Tue Jul 05 00:00:00 UTC 2022

Maternal exercise improves epithelial development of fetal intestine by enhancing apelin signaling and oxidative metabolism.

American journal of physiology. Regulatory, integrative and comparative physiology

Chae SA,Son JS,de Avila JM,Du M,Zhu MJ

PA5-20872 was used in Western Blot to suggest that maternal exercise, a highly accessible intervention, is effective in improving fetal intestinal epithelium of obese dams.

Tue Nov 01 00:00:00 UTC 2022

Exerkine apelin reverses obesity-associated placental dysfunction by accelerating mitochondrial biogenesis in mice.

American journal of physiology. Endocrinology and metabolism

Chae SA,Son JS,Zhao L,Gao Y,Liu X,Marie de Avila J,Zhu MJ,Du M

PA5-20872 was used in Western Blotting to investigate the role of maternal exercise (ME) in protecting against adverse effects of maternal obesity (MO) on fetal development.

Wed Jun 01 00:00:00 UTC 2022

Fli1⁺ cells transcriptional analysis reveals an Lmo2-Prdm16 axis in angiogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Matrone G,Xia B,Chen K,Denvir MA,Baker AH,Cooke JP

PA5-20872 was used in Western Blot to report new mechanistic insights into development of the vascular endothelium, as well as angiogenesis, focusing on the zebrafish model.

Tue Aug 03 00:00:00 UTC 2021

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Nature communications

Chen YT,Yang QY,Hu Y,Liu XD,de Avila JM,Zhu MJ,Nathanielsz PW,Du M

Published figure using PRDM16 polyclonal antibody (Product # PA5-20872) in Western Blot

Thu Nov 25 00:00:00 UTC 2021

Melatonin Regulates Differentiation of Sheep Brown Adipocyte Precursor Cells Via AMP-Activated Protein Kinase.

Frontiers in veterinary science

Gao XY,Deng BH,Li XR,Wang Y,Zhang JX,Hao XY,Zhao JX

PA5-20872 was used in Western Blotting to explore the effect and mechanism of melatonin on sheep brown adipocyte formation and function.

Fri Jul 09 00:00:00 UTC 2021

Melatonin Regulates Differentiation of Sheep Brown Adipocyte Precursor Cells Via AMP-Activated Protein Kinase.

Frontiers in veterinary science

Gao XY,Deng BH,Li XR,Wang Y,Zhang JX,Hao XY,Zhao JX

PA5-20872 was used in Western Blotting to explore the effect and mechanism of melatonin on sheep brown adipocyte formation and function.

Fri Jul 09 00:00:00 UTC 2021

Embryonic exposure to hyper glucocorticoids suppresses brown fat development and thermogenesis via REDD1.

Science bulletin

Chen YT,Hu Y,Yang QY,Liu XD,Son JS,de Avila JM,Zhu MJ,Du M

PA5-20872 was used in Western Blotting to investigate the impacts of prenatal hyper glucocorticoids on postnatal brown adipose tissue thermogenesis and underlying regulations.

Mon Mar 15 00:00:00 UTC 2021

Dietary alpha-ketoglutarate promotes beige adipogenesis and prevents obesity in middle-aged mice.

Aging cell

Tian Q,Zhao J,Yang Q,Wang B,Deavila JM,Zhu MJ,Du M

Published figure using PRDM16 polyclonal antibody (Product # PA5-20872) in Western Blot

Wed Jan 01 00:00:00 UTC 2020

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Science advances

Son JS,Zhao L,Chen Y,Chen K,Chae SA,de Avila JM,Wang H,Zhu MJ,Jiang Z,Du M

PA5-20872 was used in Western Blotting to analyze fetal brown adipose tissue (BAT) development and offspring metabolic health in mice.

Wed Apr 01 00:00:00 UTC 2020

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Science advances

Son JS,Zhao L,Chen Y,Chen K,Chae SA,de Avila JM,Wang H,Zhu MJ,Jiang Z,Du M

PA5-20872 was used in Western Blotting to analyze fetal brown adipose tissue (BAT) development and offspring metabolic health in mice.

Wed Apr 01 00:00:00 UTC 2020

Novel discovery of Averrhoa bilimbi ethanolic leaf extract in the stimulation of brown fat differentiation program in combating diet-induced obesity.

BMC complementary and alternative medicine

Lau WK,Noruddin NAA,Ariffin AH,Mahmud MZ,Noor MHM,Amanah A,Hamzah MF,Zafarina Z

PA5-20872 was used in Western Blotting to study the browning activity on white adipocytes by Averrhoa bilimbi leaf extract and evaluate the potential mechanisms.

Thu Sep 05 00:00:00 UTC 2019

Short-term thermoneutral housing alters glucose metabolism and markers of adipose tissue browning in response to a high-fat diet in lean mice.

American journal of physiology. Regulatory, integrative and comparative physiology

Clayton ZS,McCurdy CE

Published figure using PRDM16 polyclonal antibody (Product # PA5-20872) in Western Blot

Mon Oct 01 00:00:00 UTC 2018

Angiotensin type 2 receptor activation promotes browning of white adipose tissue and brown adipogenesis.

Signal transduction and targeted therapy

Than A,Xu S,Li R,Leow MK,Sun L,Chen P

Published figure using PRDM16 polyclonal antibody (Product # PA5-20872) in Western Blot

Tue Feb 02 00:00:00 UTC 2021

Resveratrol supplementation of high-fat diet-fed pregnant mice promotes brown and beige adipocyte development and prevents obesity in male offspring.

The Journal of physiology

Zou T,Chen D,Yang Q,Wang B,Zhu MJ,Nathanielsz PW,Du M

PA520872 was used in western blot to report that maternal resveratrol intervention protects offspring against high-fat diet-induced obesity

Wed Mar 01 00:00:00 UTC 2017

Alcohol intake aggravates adipose browning and muscle atrophy in cancer-associated cachexia.

Oncotarget

Wang B,Zhang F,Zhang H,Wang Z,Ma YN,Zhu MJ,Du M

PA5-20872 was used in Western Blotting to identify that elevated adipose browning and muscle atrophy through aggravated cancer-associated cachexia is observed during alcohol intake.

Tue Nov 21 00:00:00 UTC 2017

Retinoic acid induces white adipose tissue browning by increasing adipose vascularity and inducing beige adipogenesis of PDGFRα⁺ adipose progenitors.

Cell discovery

Wang B,Fu X,Liang X,Deavila JM,Wang Z,Zhao L,Tian Q,Zhao J,Gomez NA,Trombetta SC,Zhu MJ,Du M

PA5-20872 was used in Immunohistochemistry to show that retinoic acid induces white adipose tissue browning via activating vascular endothelial growth factor signalling.

Thu Oct 01 00:00:00 UTC 2020

Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes.

The Journal of biological chemistry

Than A,He HL,Chua SH,Xu D,Sun L,Leow MK,Chen P

PA5-20872 was used in western blot to investigate apelin signaling in brown adipocyte tissue

Fri Jun 05 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Histone-lysine N-methyltransferase PRDM16; line 27; MDS1/EVI1-like gene 1; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; Transcription factor MEL1

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Gene Aliases: 5730557K01Rik; CMD1LL; csp1; KIAA1675; LVNC8; MEL1; PFM13; PRDM16

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UniProt ID: (Human) Q9HAZ2, (Mouse) A2A935

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Entrez Gene ID: (Human) 63976, (Mouse) 70673

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Function(s)

transcription coactivator activity protein binding histone-lysine N-methyltransferase activity activating transcription factor binding sequence-specific DNA binding SMAD binding metal ion binding nucleic acid binding DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated stem cell population maintenance neurogenesis cell differentiation negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of granulocyte differentiation somatic stem cell population maintenance regulation of cellular respiration tongue development negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated white fat cell differentiation brown fat cell differentiation palate development positive regulation of brown fat cell differentiation histone lysine methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRDM16 Polyclonal Antibody

PRDM16 Antibody (PA5-20872) in ICC/IF

Product Details

PA5-20872

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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