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Invitrogen
PRDX6 Monoclonal Antibody (6I8)
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FIGURE: 1 / 5
PRDX6 Antibody (MA5-49212) in ICC/IF
Immunocytochemistry analysis of PRDX6 in A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PRDX6 Monoclonal antibody (Product # MA5-49212) using a dilution of 2 µg/mL. Cy3 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the lab... View More
Product Details
MA5-49212
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
6I8
Immunogen
E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074870
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively.
Positive Control - WB: human Hela tissue, human Jurkat whole cell, human HEK293 whole cell, human K562 whole cell, human SW620 whole cellrat RH35 whole cell. IHC: human endometrial carcinoma tissue. ICC/IF: A549 Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins The cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; epididymis secretory sperm binding protein Li 128m; Glutathione-dependent peroxiredoxin; Liver 2D page spot 40; LPC acyltransferase 5; Lysophosphatidylcholine acyltransferase 5; MGC46173; MSP23; NKEFA; Non-selenium glutathione peroxidase; NSGPx; PAG; PAGA; PAGB; Peroxiredoxin-6; Prx6; Red blood cells page spot 12; Thiol-specific antioxidant protein
Gene Aliases: 1-Cys; 1-Cys Prx; 1-cysPrx; 9430088D19Rik; AA690119; Aipla2; AOP2; Brp-12; CP-3; GPx; HEL-S-128m; KIAA0106; Ltw-4; Ltw4; Lvtw-4; NSGPx; ORF06; p29; Prdx5; PRDX6; PRX; Tsa
UniProt ID: (Human) P30041, (Mouse) O08709, (Rat) O35244
Entrez Gene ID: (Human) 9588, (Mouse) 11758, (Rat) 94167
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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