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Invitrogen
PREP Polyclonal Antibody
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FIGURE: 1 / 3
PREP Antibody (PA5-115798) in ICC/IF
Immunocytochemistry analysis of PREP in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115798) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115798
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PREP(Accession P48147), corresponding to amino acid residues L168-D218.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900433
Product Specific Information
Antibody detects endogenous levels of total Prolyl Endopeptidase.
Target Information
p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dJ355L5.1 (prolyl endopeptidase); EC 3.4.21.26; MGC16060; PE; PE {ECO:0000250|UniProtKB:P23687}; Post-proline cleaving enzyme; post-proline cleaving enzyme {ECO:0000250|UniProtKB:P23687}; prep {ECO:0000312|RGD:620841}; Prolyl endopeptidase; prolyl endopeptidase {ECO:0000250|UniProtKB:P23687}; prolyl oligopeptidase; rPop
Gene Aliases: AI047692; AI450383; D10Wsu136e; PE; PEP; Pop; PREP; rPop
UniProt ID: (Human) P48147, (Mouse) Q9QUR6, (Rat) O70196
Entrez Gene ID: (Human) 5550, (Mouse) 19072, (Rat) 83471
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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