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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PRIM1 Antibodies

          Proteintech

          PRIM1 Polyclonal Antibody

          5 References
          View all (6) PRIM1 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite PRIM1 Polyclonal Antibody

          • Antibody Testing Data (10)
          PRIM1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PRIM1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          PRIM1 Antibody (10773-1-AP) in ICC/IF

          Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10773-1-AP (PRIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PRIM1 Antibody in Immunocytochemistry (ICC/IF)
          PRIM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PRIM1 Antibody in Western Blot (WB)
          PRIM1 Antibody in Western Blot (WB)
          PRIM1 Antibody in Western Blot (WB)
          PRIM1 Antibody in Western Blot (WB)
          PRIM1 Antibody in Western Blot (WB)
          PRIM1 Antibody in Western Blot (WB)
          PRIM1 Antibody in Western Blot (WB)
          PRIM1 Antibody in Immunoprecipitation (IP)

          Product Details

          10773-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          PRIM1 Fusion Protein Ag1124 (121-420 aa encoded by BC005266)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.13 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: CCSSADICP KCWTLMTMAI RIIDRALKED FGFKHRLWVY SGRRGVHCWV CDESVRKLSS AVRSGIVEYL SLVKGGQDVK KKVHLSEKIH PFIRKSINII KKYFEEYALV NQDILENKES WDKILALVPE TIHDELQQSF QKSHNSLQRW EHLKKVASRY QNNIKNDKYG PWLEWEIMLQ YCFPRLDINV SKGINHLLKS PFSVHPKTGR ISVPIDLQKV DQFDPFTVPT ISFICRELDA ISTNEEEKEE NEAESDVKHR TRDYKKTSLA PYVKVFEHFL ENLDKSRKGE LLKKSDLQKD F (121-420 aa encoded by BC005266)

          Target Information

          The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DNA primase 1; DNA primase 49 kDa subunit; DNA primase small subunit; DNA primase subunit 48; MGC12308; p49; PRI1; primase p49 subunit; primase polypeptide 1, 49kDa; primase, DNA, polypeptide 1 (49kDa)

          View more View less

          Gene Aliases: AI324982; p49; PRIM1

          View more View less

          UniProt ID: (Human) P49642, (Mouse) P20664

          View more View less

          Entrez Gene ID: (Human) 5557, (Mouse) 19075, (Rat) 246327

          View more View less

          Function(s)
          DNA primase activity metal ion binding telomerase activity RNA helicase activity DNA-directed RNA polymerase activity sulfate adenylyltransferase activity protein binding RNA guanylyltransferase activity tRNA guanylyltransferase activity mannose-phosphate guanylyltransferase activity transferase activity nucleotidyltransferase activity DNA/RNA helicase activity CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity phospholactate guanylyltransferase activity ATP:coenzyme F420 adenylyltransferase activity UDP-N-acetylgalactosamine diphosphorylase activity
          Process(es)
          DNA replication DNA replication, synthesis of RNA primer transcription, DNA-templated G1/S transition of mitotic cell cycle telomere maintenance via recombination DNA replication initiation DNA strand elongation involved in DNA replication
          It has to be done as per old AB suggested Products section.
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