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Invitrogen
PRIM1 Polyclonal Antibody
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PRIM1 Antibody (PA5-106653) in IHC (P)
Immunohistochemistry analysis of PRIM1 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PRIM1 polyclonal antibody (Product # PA5-106653) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-106653
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PRIM1(Accession P49642), corresponding to amino acid residues T380-F420.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854321
Product Specific Information
Antibody detects endogenous levels of total PRIM1.
Target Information
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA primase 1; DNA primase 49 kDa subunit; DNA primase small subunit; DNA primase subunit 48; MGC12308; p49; PRI1; primase p49 subunit; primase polypeptide 1, 49kDa; primase, DNA, polypeptide 1 (49kDa)
Gene Aliases: AI324982; p49; PRIM1
UniProt ID: (Human) P49642, (Mouse) P20664
Entrez Gene ID: (Human) 5557, (Mouse) 19075, (Rat) 246327
DNA primase activity
metal ion binding
telomerase activity
RNA helicase activity
DNA-directed RNA polymerase activity
sulfate adenylyltransferase activity
protein binding
RNA guanylyltransferase activity
tRNA guanylyltransferase activity
mannose-phosphate guanylyltransferase activity
transferase activity
nucleotidyltransferase activity
DNA/RNA helicase activity
CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity
phospholactate guanylyltransferase activity
ATP:coenzyme F420 adenylyltransferase activity
UDP-N-acetylgalactosamine diphosphorylase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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