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Invitrogen
PRIM2 Polyclonal Antibody
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FIGURE: 1 / 2
PRIM2 Antibody (PA5-88189) in WB
Western blot analysis of PRIM2 in extracts of various cell lines. Samples were incubated with PRIM2 Polyclonal antibody (Product # PA5-88189) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-88189
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_0009382)
Conjugate
Form
Liquid
Concentration
0.89 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804729
Product Specific Information
Immunogen sequence: MEFSGRKWRK LRLAGDQRNA SYPHCLQFYL QPPSENISLI EFENLAIDRV KLLKSVENLG VSYVKGTEQY QSKLESELRK LKFSYRENLE DEYEPRRRDH ISHFILRLAY CQSEELRRWF IQQEMDLLRF RFSILPKDKI QDFLKDSQLQ FEAISDEEKT LREQEIVASS; Positive Samples: A375, H460, HeLa, HT-29, Mouse spleen
Target Information
DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA primase 58 kDa subunit; DNA primase large subunit; p58; primase, DNA, polypeptide 2 (58kDa)
Gene Aliases: AI323589; p58; PRIM2; PRIM2A
UniProt ID: (Human) P49643, (Mouse) P33610
Entrez Gene ID: (Human) 5558, (Mouse) 19076
DNA binding
DNA primase activity
metal ion binding
4 iron, 4 sulfur cluster binding
telomerase activity
RNA helicase activity
DNA-directed RNA polymerase activity
sulfate adenylyltransferase activity
RNA guanylyltransferase activity
tRNA guanylyltransferase activity
mannose-phosphate guanylyltransferase activity
transferase activity
nucleotidyltransferase activity
DNA/RNA helicase activity
CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity
phospholactate guanylyltransferase activity
ATP:coenzyme F420 adenylyltransferase activity
iron-sulfur cluster binding
UDP-N-acetylgalactosamine diphosphorylase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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