Proteintech

PRKG1 Polyclonal Antibody

PRKG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PRKG1 Antibody (21646-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using (Product # 21646-1-AP) (PRKG1 Antibody) at dilution of 1:200 (under 10x lens).

PRKG1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21646-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PRKG1 Fusion Protein Ag16333 (1-178 aa encoded by BC127090)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGTLRDLQY ALQEKIEELR QRDALIDELE LELDQKDELI QKLQNELDKY RSVIRPATQQ AQKQSASTLQ GEPRTKRQAI SAEPTAFDIQ DLSHVTLPFY PKSPQSKDLI KEAILDNDFM KNLELSQIQE IVDCMYPVEY GKDSCIIKEG DVGSLVYVME DGKVEVTKEG VKLCTMGPG (1-178 aa encoded by BC127090 )

Target Information

PRKG1 is a cGMP-dependent serine/threonine kinase which has an important role in the relaxation of vascular smooth muscle and the inhibition of platelet aggregation. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC (soluble guanylate cyclase) - cGMP (cyclic guanosine monophosphate) - PKG (protein kinase G) pathway.

Journal of ethnopharmacology

Chen L,Zhou X,Deng Y,Yang Y,Chen X,Chen Q,Liu Y,Fu X,Kwan HY,You Y,Jin W,Zhao X

Tue Jan 10 00:00:00 UTC 2023

Specific dilation pattern in placental circulation and the NO/sGC role in preeclampsia placental vessels.

Frontiers in endocrinology

Tang J,Zhang Y,Zhang Z,Tao J,Wu J,Zheng Q,Xu T,Li N,Xu Z

Mon Jun 12 00:00:00 UTC 2023

A novel phosphodiesterase 9A inhibitor LW33 protects against ischemic stroke through the cGMP/PKG/CREB pathway.

European journal of pharmacology

You JY,Liu XW,Bao YX,Shen ZN,Wang Q,He GY,Lu J,Zhang JG,Chen JW,Liu PQ

Wed Jun 15 00:00:00 UTC 2022

Potential cardiotoxicity induced by Euodiae Fructus: In vivo and in vitro experiments and untargeted metabolomics research.

Frontiers in pharmacology

Zhang D,Lü J,Ren Z,Zhang X,Wu H,Sa R,Wang X,Wang Y,Lin Z,Zhang B

Sat Sep 07 00:00:00 UTC 2024

NEK7 promotes gastric cancer progression as a cell proliferation regulator.

Cancer cell international

Li YK,Zhu XR,Zhan Y,Yuan WZ,Jin WL

Sat Aug 21 00:00:00 UTC 2021

Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.

Frontiers in cell and developmental biology

Gu M,Zhou X,Zhu L,Gao Y,Gao L,Bai C,Yang L,Li G

Wed Feb 16 00:00:00 UTC 2022

Emodin Interferes With Nitroglycerin-Induced Migraine in Rats Through CGMP-PKG Pathway.

Frontiers in pharmacology

Sun S,Zheng G,Zhou D,Zhu L,He X,Zhang C,Wang C,Yuan C

Wed Nov 10 00:00:00 UTC 2021

Abnormal cGMP-dependent protein kinase I-mediated decidualization in preeclampsia.

Hypertension research : official journal of the Japanese Society of Hypertension

Chen J,Ren W,Lin L,Zeng S,Huang L,Tang J,Bi S,Pan J,Chen D,Du L

Mon Mar 01 00:00:00 UTC 2021

Abnormal cGMP-dependent protein kinase I-mediated decidualization in preeclampsia.

Hypertension research : official journal of the Japanese Society of Hypertension

Chen J,Ren W,Lin L,Zeng S,Huang L,Tang J,Bi S,Pan J,Chen D,Du L

Mon Mar 01 00:00:00 UTC 2021

Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway.

World neurosurgery

Lan SH,Lai WT,Zheng SY,Yang L,Fang LC,Zhou L,Tang B,Duan J,Hong T

Wed Apr 01 00:00:00 UTC 2020

Long non-coding RNA and mRNA profile analysis of metformin to reverse the pulmonary hypertension vascular remodeling induced by monocrotaline.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Sun Z,Liu Y,Yu F,Xu Y,Yanli L,Liu N

Mon Jul 01 00:00:00 UTC 2019

Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.

Environmental pollution (Barking, Essex : 1987)

Han Y,Yu Y,Liang C,Shi Y,Zhu Y,Zheng H,Wang J,Zhang J

Tue Oct 01 00:00:00 UTC 2019

Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.

Clinical science (London, England : 1979)

Xue M,Li T,Wang Y,Chang Y,Cheng Y,Lu Y,Liu X,Xu L,Li X,Yu X,Sun B,Chen L

Thu Aug 15 00:00:00 UTC 2019

Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro.

Oncotarget

Zhu E,Yang Y,Zhang J,Li Y,Li C,Chen L,Sun B

Tue Dec 06 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: cGK 1; cGK cGK cGKI; cGK cGK1; cGK1; cGKI; cGMP dependent protein kinase type 1; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase I; OTTHUMP00000019618; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I; RP11-346D6.1

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Gene Aliases: AAT8; AW125416; cGK; cGK 1; cGK1; CGKI; cGKI-alpha; cGKI-BETA; Gm19690; PKG; Pkgi; PRKG1; PRKG1B; PRKGR1A; PRKGR1B

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UniProt ID: (Human) Q13976, (Mouse) P0C605

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Entrez Gene ID: (Human) 5592, (Mouse) 19091, (Rat) 54286

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Function(s)

protein serine/threonine kinase activity cGMP-dependent protein kinase activity calcium channel regulator activity protein binding ATP binding cGMP binding nucleotide binding catalytic activity protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein homodimerization activity mitogen-activated protein kinase p38 binding

Process(es)

neuron migration protein phosphorylation signal transduction positive regulation of cytosolic calcium ion concentration metabolic process negative regulation of inositol phosphate biosynthetic process negative regulation of glutamate secretion negative regulation of smooth muscle cell migration phosphorylation dendrite development cGMP-mediated signaling forebrain development positive regulation of circadian rhythm regulation of GTPase activity negative regulation of heart contraction positive regulation of vasodilation negative regulation of smooth muscle contraction relaxation of vascular smooth muscle cell growth involved in cardiac muscle cell development negative regulation of platelet aggregation mitophagy in response to mitochondrial depolarization positive regulation of large conductance calcium-activated potassium channel activity regulation of testosterone biosynthetic process spermatid development brain development heart development cerebellum development actin cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRKG1 Polyclonal Antibody

PRKG1 Antibody (21646-1-AP) in IHC (P)

Product Details

21646-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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