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Invitrogen
PRMT4 Polyclonal Antibody
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FIGURE: 1 / 4
PRMT4 Antibody (PA5-78913) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of PRMT4 in Hela cells using PRMT4 Polyclonal Antibody (Product # PA5-78913). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter set... View More
Product Details
PA5-78913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PRMT4 (267-284aa HLAPFTDEQLYMEQFTKA).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746029
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell, rat testis tissue, rat C6 whole cell.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coactivator-associated arginine methyltransferase 1; coactivator-associated arginine methyltransferase 1 variant 5; Histone-arginine methyltransferase CARM1; Protein arginine N-methyltransferase 4
Gene Aliases: CARM1; PRMT4
UniProt ID: (Human) Q86X55, (Rat) Q4AE70
Entrez Gene ID: (Human) 10498, (Rat) 363026
transcription coactivator activity
protein binding
beta-catenin binding
protein methyltransferase activity
histone-arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
ligand-dependent nuclear receptor transcription coactivator activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity (H3-R17 specific)
histone methyltransferase activity
protein homodimerization activity
transcription regulatory region DNA binding
lysine-acetylated histone binding
regulation of growth plate cartilage chondrocyte proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
aging
positive regulation of cell proliferation
viral process
histone methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
intracellular estrogen receptor signaling pathway
negative regulation of protein binding
regulation of intracellular estrogen receptor signaling pathway
histone H3-R2 methylation
histone H3-R17 methylation
cellular lipid metabolic process
positive regulation of fat cell differentiation
response to cAMP
endochondral bone morphogenesis
regulation of mRNA binding
negative regulation of dendrite development
mRNA processing
protein methylation
RNA splicing
pathogenesis
intracellular steroid hormone receptor signaling pathway
histone arginine methylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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