Proteintech

PRMT6 Polyclonal Antibody

PRMT6 Antibody in Immunocytochemistry (ICC/IF)

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PRMT6 Antibody (15395-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 15395-1-AP (PRMT6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PRMT6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT6 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15395-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PRMT6 Fusion Protein Ag7592 (1-316 aa encoded by BC002729)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Western blot analysis using this antibody detected a major band around 42 kDa in Hela cells.

Immunogen sequence: MIADRVRTD AYRLGILRNW AALRGKTVLD VGAGTGILSI FCAQAGARRV YAVEASAIWQ QAREVVRFNG LEDRVHVLPG PVETVELPEQ VDAIVSEWMG YGLLHESMLS SVLHARTKWL KEGGLLLPAS AELFIAPISD QMLEWRLGFW SQVKQHYGVD MSCLEGFATR CLMGHSEIVV QGLSGEDVLA RPQRFAQLEL SRAGLEQELE AGVGGRFRCS CYGSAPMHGF AIWFQVTFPG GESEKPLVLS TSPFHPATHW KQALLYLNEP VQVEQDTDVS GEITLLPSRD NPRRLRVLLR YKVGDQEEKT KDFAMED (1-316 aa encoded by BC002729)

Target Information

Protein arginine N-methyltransferase 6 (PRMT6) PRMT6 demonstrates type I PRMT activity, capable of forming both omega-N monomethylarginine and asymmetric dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. cts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice.

Nature communications

Prakasam R,Bonadiman A,Andreotti R,Zuccaro E,Dalfovo D,Marchioretti C,Tripathy D,Petris G,Anderson EN,Migazzi A,Tosatto L,Cereseto A,Battaglioli E,Sorarù G,Lim WF,Rinaldi C,Sambataro F,Pourshafie N,Grunseich C,Romanel A,Pandey UB,Contestabile A,Ronzitti G,Basso M,Pennuto M

Mon Feb 06 00:00:00 UTC 2023

LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice.

Nature communications

Mon Feb 06 00:00:00 UTC 2023

Disrupted liver oxidative metabolism in glycine N-methyltransferase-deficient mice is mitigated by dietary methionine restriction.

Molecular metabolism

Rome FI,Hughey CC

Fri Apr 01 00:00:00 UTC 2022

F-Box Protein FBXW17-Mediated Proteasomal Degradation of Protein Methyltransferase PRMT6 Exaggerates CSE-Induced Lung Epithelial Inflammation and Apoptosis.

Frontiers in cell and developmental biology

Li T,He X,Luo L,Zeng H,Ren S,Chen Y

Sat May 08 00:00:00 UTC 2021

Huntingtin-mediated axonal transport requires arginine methylation by PRMT6.

Cell reports

Migazzi A,Scaramuzzino C,Anderson EN,Tripathy D,Hernández IH,Grant RA,Roccuzzo M,Tosatto L,Virlogeux A,Zuccato C,Caricasole A,Ratovitski T,Ross CA,Pandey UB,Lucas JJ,Saudou F,Pennuto M,Basso M

Tue Apr 13 00:00:00 UTC 2021

Generation of PRMT6 homozygous knockout human embryonic stem cell lines.

Stem cell research

Li T,Jin W,He Y,Zhou Z

Mon Dec 28 00:00:00 UTC 2020

Targeting G6PD reverses paclitaxel resistance in ovarian cancer by suppressing GSTP1.

Biochemical pharmacology

Feng Q,Li X,Sun W,Sun M,Li Z,Sheng H,Xie F,Zhang S,Shan C

Sat Aug 01 00:00:00 UTC 2020

Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling.

Aging

Li T,Fanning KV,Nyunoya T,Chen Y,Zou C

Tue Dec 01 00:00:00 UTC 2020

Arginine methylation of SIRT7 couples glucose sensing with mitochondria biogenesis.

EMBO reports

Yan WW,Liang YL,Zhang QX,Wang D,Lei MZ,Qu J,He XH,Lei QY,Wang YP

Sat Dec 01 00:00:00 UTC 2018

Arginine methylation of SIRT7 couples glucose sensing with mitochondria biogenesis.

EMBO reports

Yan WW,Liang YL,Zhang QX,Wang D,Lei MZ,Qu J,He XH,Lei QY,Wang YP

Sat Dec 01 00:00:00 UTC 2018

Arginine methylation of SIRT7 couples glucose sensing with mitochondria biogenesis.

EMBO reports

Yan WW,Liang YL,Zhang QX,Wang D,Lei MZ,Qu J,He XH,Lei QY,Wang YP

Sat Dec 01 00:00:00 UTC 2018

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

PLoS pathogens

Xu M,Moresco JJ,Chang M,Mukim A,Smith D,Diedrich JK,Yates JR,Jones KA

Tue May 01 00:00:00 UTC 2018

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

PLoS pathogens

Xu M,Moresco JJ,Chang M,Mukim A,Smith D,Diedrich JK,Yates JR,Jones KA

Tue May 01 00:00:00 UTC 2018

PRMT6 mediates CSE induced inflammation and apoptosis.

International immunopharmacology

Kang N,Chen P,Chen Y,Zeng H,He X,Zhu Y

Thu Jan 01 00:00:00 UTC 2015

PRMT6 mediates CSE induced inflammation and apoptosis.

International immunopharmacology

Kang N,Chen P,Chen Y,Zeng H,He X,Zhu Y

Thu Jan 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: FLJ10559; FLJ51477; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase-like 6; PRMT-6; Protein arginine N-methyltransferase 6

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Gene Aliases: AW124876; BB233495; HRMT1L6; PRMT6

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UniProt ID: (Human) Q96LA8, (Mouse) Q6NZB1

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Entrez Gene ID: (Human) 55170, (Mouse) 99890

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Function(s)

Process(es)

negative regulation of transcription from RNA polymerase II promoter DNA repair transcription, DNA-templated regulation of transcription, DNA-templated protein methylation cellular response to DNA damage stimulus cell growth chromatin modification histone methylation peptidyl-arginine methylation, to asymmetrical-dimethyl arginine methylation histone arginine methylation histone H3-R2 methylation peptidyl-arginine N-methylation histone H4-R3 methylation negative regulation of transcription, DNA-templated cellular senescence base-excision repair viral process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRMT6 Polyclonal Antibody

PRMT6 Antibody (15395-1-AP) in ICC/IF

Product Details

15395-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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