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PROP1 Antibody (PA5-116708) in WB
Western blot analysis of PROP1 in mouse brain. Samples were incubated with PROP1 Polyclonal antibody (Product # PA5-116708) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 50s.
Product Details
PA5-116708
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PROP1 (NP_0062523)
Conjugate
Form
Liquid
Concentration
0.89 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901339
Product Specific Information
Positive Samples: Mouse brain
Immunogen sequence: GGGRSRFSPQ GGQRGRPHSR RRHRTTFSPV QLEQLESAFG RNQYPDIWAR ESLARDTGLS EARIQVWFQN RRAKQRKQER SLLQPLAHLS PAAFSSFLPE S
Target Information
PROP1 encodes a paired-like homeodomain transcription factor in the developing pituitary gland. Expression occurs prior to and is required for expression of pou domain transcription factor 1, which is responsible for pituitary development and hormone expression. Mutations in this gene have been associated with combined pituitary hormone deficiency-2 as well as deficiencies in luteinizing hormone, follicle-stimulating hormone, growth hormone, prolactin, and thyroid-stimulating hormone.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ames dwarf; Homeobox protein prophet of Pit-1; pituitary specific homeodomain factor; Pituitary-specific homeodomain factor; PROP-1; prophet of Pit-1
Gene Aliases: df; Prop-1; Prop1
UniProt ID: (Mouse) P97458
Entrez Gene ID: (Mouse) 19127
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding
DNA binding
chromatin binding
protein binding
beta-catenin binding
protein C-terminus binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
organ morphogenesis
dorsal/ventral pattern formation
cell migration
hypothalamus cell differentiation
adenohypophysis development
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
gland development
hypophysis morphogenesis
canonical Wnt signaling pathway
somatotropin secreting cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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