Proteintech

PROX1 Monoclonal Antibody (1D4B1), CoraLite® Plus 488

View all (18) PROX1 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CL488-67438

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:50-1:500
-

Flow Cytometry (Flow)

0.4 µg/1x10^6 cell suspension
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1D4B1

Immunogen

Recombinant protein
View immunogen

Conjugate

CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488

Excitation/Emission Max

493/522 nm

Form

Liquid

Purification

Protein A

Storage buffer

PBS with 0.5% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, store in dark

Shipping conditions

Wet ice

Product Specific Information

Immunogen sequence: FHTANQRLQC FGDVIIPNPL DTFGNVQMAS STDQTEALPL VVRKNSSDQS ASGPAAGGHH QPLHQSPLSA TTGFTTSTFR HPFPLPLMAY PFQSPLGAPS GSFSGKDRAS PESLDLTRDT TSLRTKMSSH HLSHHPCSPA HPPSTAEGLS LSLIKSECGD LQDMSEISPY SGSAMQEGLS PNHLKKAKLM FFYTRYPSSN MLKTYFSDVK FNRCITSQLI KWFSNFREFY YIQMEKYARQ AINDGVTSTE ELSITRDCEL YRALNMHYNK ANDFEVPERF LEVAQITLRE FFNAIIAGKD VDPSWKKAIY KVICKLDSEV PEIFKSPNCL QELLHE

Target Information

The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: Homeobox prospero-like protein PROX1; Prospero homeobox protein 1; prospero-related homeobox 1; PROX-1

View more View less

Gene Aliases: A230003G05Rik; PROX1

View more View less

UniProt ID: (Human) Q92786, (Mouse) P48437

View more View less

Entrez Gene ID: (Human) 5629, (Rat) 305066, (Mouse) 19130

View more View less

Function(s)
core promoter sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding ligand-dependent nuclear receptor binding transcription regulatory region DNA binding DBD domain binding LBD domain binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cell fate determination kidney development liver development positive regulation of endothelial cell proliferation lymphangiogenesis lens development in camera-type eye hepatocyte cell migration transcription, DNA-templated brain development circadian rhythm positive regulation of cell proliferation negative regulation of cell proliferation regulation of gene expression positive regulation of endothelial cell migration dorsal spinal cord development dentate gyrus development cerebellar granule cell differentiation neural tube development neuron differentiation skeletal muscle thin filament assembly lung development olfactory placode formation pancreas development response to nutrient levels regulation of circadian rhythm otic placode formation negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of viral genome replication positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter optic placode formation involved in camera-type eye formation venous blood vessel morphogenesis ventricular cardiac myofibril assembly cardiac muscle cell differentiation atrial cardiac muscle tissue morphogenesis ventricular cardiac muscle tissue morphogenesis retina morphogenesis in camera-type eye embryonic retina morphogenesis in camera-type eye endocardium formation positive regulation of sarcomere organization ventricular septum morphogenesis aorta smooth muscle tissue morphogenesis positive regulation of heart growth lymphatic endothelial cell differentiation regulation of transcription involved in lymphatic endothelial cell fate commitment branching involved in pancreas morphogenesis lens fiber cell morphogenesis hepatocyte differentiation negative regulation of bile acid biosynthetic process hepatocyte proliferation acinar cell differentiation neuronal stem cell population maintenance positive regulation of cell cycle checkpoint positive regulation of neural precursor cell proliferation positive regulation of forebrain neuron differentiation lymph vessel development lens morphogenesis in camera-type eye transcription from RNA polymerase II promoter endothelial cell differentiation inner ear development regulation of transcription, DNA-templated multicellular organism development rhythmic process
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support