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Invitrogen
PRPF18 Polyclonal Antibody
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FIGURE: 1 / 1
PRPF18 Antibody (PA5-88776) in WB
Western blot analysis of PRPF18 in extracts of HeLa cells. Samples were incubated with PRPF18 Polyclonal antibody (Product # PA5-88776) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88776
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_0036661)
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805123
Product Specific Information
Immunogen sequence: MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL; Positive Samples: HeLa; Cellular Location: Nucleus speckle
Target Information
PRPF18 (pre-mRNA-splicing factor 18), also known as HPRP18, is a 342 amino acid protein that localizes to nuclear speckles and plays a role in the second step of pre-mRNA splicing. A member of the PRP18 family, PRPF18 contains seven WD repeats and exists as two alternatively spliced isoforms which are encoded by a gene located on human chromosome 10p13. Chromosome 10 contains over 800 genes and 135 million nucleotides. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome. Other chromosome 10 associated disorders include Cockayne syndrome, tetrahydrobiopterin deficiency and trisomy 10.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hPRP18; Pre-mRNA-splicing factor 18; PRP18 homolog; PRP18 pre-mRNA processing factor 18 homolog; PRP18 pre-mRNA processing factor 18 homolog(PRPF18)
Gene Aliases: HPRP18; PRP18; PRPF18
UniProt ID: (Human) Q99633
Entrez Gene ID: (Human) 8559
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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