Invitrogen

PRR11 Polyclonal Antibody

1 Published Figure

View all (8) PRR11 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (4)
Published Figures (1)

PRR11 Antibody in Immunocytochemistry (ICC/IF)

PRR11 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

PRR11 Antibody (PA5-113175) in ICC/IF

Immunocytochemichal analysis of PRR11 in PC-3 cells using a Polyclonal antibody (Product # PA5-113175) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

PRR11 Antibody in Immunocytochemistry (ICC/IF)

PRR11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRR11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRR11 Antibody in Western Blot (WB)

PRR11 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

Product Image Gallery

PRR11 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemichal analysis of PRR11 in PC-3 cells using a Polyclonal antibody (Product # PA5-113175) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

PRR11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded PRR11 in human lung cancer tissue using a PRR11 polyclonal antibody (Product # PA5-113175) at a dilution of 1:100.

PRR11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded PRR11 in human pancreatic cancer using a PRR11 Polyclonal antibody (Product # PA5-113175) at a dilution of 1:100.

PRR11 Antibody in Western Blot (WB) — Western blot analysis of PRR11 using a polyclonal antibody (Product # PA5-113175) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in mouse heart tissue. Observed band: 41 kDa.

PRR11 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP) — FIGURE 6 PRR11 promotes the expression level of PTTG1 by interacting with E2F1. (A) Localization for PRR11 gene was analyzed using GeneCards ( https://www.genecards.org/ ). (B) Immunofluorescence localization of PRR11. (C) Expression levels of PTTG1 after interfering E2F1. (D) ChIP-qPCR of the PTTG1 promoter region. (E) Expression levels of E2F1 and PRR11 in NCI-H460 cells transfected with siNC or siPRR11 were examined by Western blotting. (F) Co-IP of PRR11 and E2F1 in NCI-H460 cells. N = 3. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Product Details

PA5-113175

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:5,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Proline-rich protein 11 protein (1-360AA)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% ProClin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2867909

Target Information

Plays a critical role in cell cycle progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

PA5-113175 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to find that PRR11 served as an oncogene in pan-cancer and influences via cell cycle progression by PTTG1 expression regulation, through TF E2F1 interaction.

Wed Sep 07 00:00:00 UTC 2022

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

PA5-113175 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to find that PRR11 served as an oncogene in pan-cancer and influences via cell cycle progression by PTTG1 expression regulation, through TF E2F1 interaction.

Wed Sep 07 00:00:00 UTC 2022

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

PA5-113175 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to find that PRR11 served as an oncogene in pan-cancer and influences via cell cycle progression by PTTG1 expression regulation, through TF E2F1 interaction.

Wed Sep 07 00:00:00 UTC 2022

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

PA5-113175 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to find that PRR11 served as an oncogene in pan-cancer and influences via cell cycle progression by PTTG1 expression regulation, through TF E2F1 interaction.

Wed Sep 07 00:00:00 UTC 2022

PRR11 promotes cell proliferation by regulating PTTG1 through interacting with E2F1 transcription factor in pan-cancer.

Frontiers in molecular biosciences

Zhang H,He Z,Qiu L,Wei J,Gong X,Xian M,Chen Z,Cui Y,Fu S,Zhang Z,Hu B,Zhang X,Lin S,Du H

PA5-113175 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to find that PRR11 served as an oncogene in pan-cancer and influences via cell cycle progression by PTTG1 expression regulation, through TF E2F1 interaction.

Wed Sep 07 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: Proline-rich protein 11; transcription repressor of MHCII

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Gene Aliases: B930067F20Rik; PRR11

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UniProt ID: (Human) Q96HE9, (Mouse) Q8BHE0

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Entrez Gene ID: (Human) 55771, (Mouse) 270906

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molecular_function

regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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