Invitrogen

PSA-NCAM Monoclonal Antibody (12E3), eBioscience™

PSA-NCAM Antibody in Immunocytochemistry (ICC/IF)

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PSA-NCAM Antibody (14-9118-82) in ICC/IF

Immunofluorescence analysis of PSA-NCAM was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSA-NCAM Monoclonal Antibody (12E3), eBioscience (Product # 14-9118-82) at 20 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... View More

PSA-NCAM Antibody in Immunohistochemistry (IHC)

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Product Details

14-9118-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

20 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

12E3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572932

Product Specific Information

Description: The monoclonal antibody 12E3 specifically recognizes polysialylated neural cell adhesion molecule (PSA-NCAM) in human, mouse and rat. Polysialic acid is a long homopolymer of sialic acid that is negatively charged and is attached to neural cell adhesion molecule (CD56) and serves as a regulator of NCAM function. PSA attachment to NCAM is associated with inhibited adhesion of neural cells. PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows in which neural precursors are migrating and during the process of axonal sprouting, guidance, and targeting. PSA-NCAM expression is prevalent during development of the brain, but in the adult becomes restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and specific spinal cord nuclei. PSA-NCAM is re-expressed during tumorigenesis and is also expressed on cell lines isolated from neuroblastomas and pheochromocytomas.

Applications Reported: This 12E3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 12E3 antibody has been tested by immunocytochemistry of formaldehyde-fixed PC-12 (rat) cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Polysialic acid is a long homopolymer of sialic acid that is negatively charged and is attached to neural cell adhesion molecule (CD56) and serves as a regulator of NCAM function. PSA attachment to NCAM is associated with inhibited adhesion of neural cells. PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows in which neural precursors are migrating and during the process of axonal sprouting, guidance, and targeting. PSA-NCAM expression is prevalent during development of the brain, but in the adult becomes restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and specific spinal cord nuclei. PSA-NCAM is re-expressed during tumorigenesis and is also expressed on cell lines isolated from neuroblastomas and pheochromocytomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Experimental Treatment with Edaravone in a Mouse Model of Spinocerebellar Ataxia 1.

International journal of molecular sciences

Sucha M,Benediktova S,Tichanek F,Jedlicka J,Kapl S,Jelinkova D,Purkartova Z,Tuma J,Kuncova J,Cendelin J

14-9118-82 was used in Immunohistochemistry to explore the potential of edaravone to slow down SCA1 progression in a mouse knock-in SCA1 model.

Mon Jun 26 00:00:00 UTC 2023

Peak density of immature nerve cells occurs with high-grade dysplasia in intraductal papillary mucinous neoplasms of the pancreas.

The Journal of pathology

Trinh VQ,Roland JT,Wong J,Revetta F,Patel K,Shi C,DelGiorno KE,Carter BD,Tan MCB

14-9118-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to investigate neoplasia in nerves in resected intraductal papillary mucinous neoplasms of the pancreas.

Thu Sep 01 00:00:00 UTC 2022

Neuroblasts contribute to oligodendrocytes generation upon demyelination in the adult mouse brain.

iScience

El Waly B,Bertet C,Paris M,Falque M,Milpied P,Magalon K,Cayre M,Durbec P

14-9118-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to demonstrate that SVZ neuroblasts fate conversion proceeds through formation of a non-proliferating transient cellular state co-expressing markers of both neuronal and oligodendrocyte identities.

Fri Oct 21 00:00:00 UTC 2022

Neurogenesis of medium spiny neurons in the nucleus accumbens continues into adulthood and is enhanced by pathological pain.

Molecular psychiatry

García-González D,Dumitru I,Zuccotti A,Yen TY,Herranz-Pérez V,Tan LL,Neitz A,García-Verdugo JM,Kuner R,Alfonso J,Monyer H

14-9118-82 was used in Immunohistochemistry to find that the number of newborn neurons in the NAc is dynamically regulated by persistent pain, suggesting that adult neurogenesis of MSNs is an experience-modulated process.

Wed Sep 01 00:00:00 UTC 2021

Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer's Disease Mice.

International journal of molecular sciences

Polis B,Srikanth KD,Gurevich V,Bloch N,Gil-Henn H,Samson AO

Published figure using PSA-NCAM monoclonal antibody (Product # 14-9118-82) in Immunohistochemistry

Sat Feb 08 00:00:00 UTC 2020

Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.

Molecular & cellular proteomics : MCP

Nakano M,Mishra SK,Tokoro Y,Sato K,Nakajima K,Yamaguchi Y,Taniguchi N,Kizuka Y

14-9118 was used in Immunohistochemistry-immunofluorescence to unveil a new regulation mechanism of protein N-glycosylation.

Tue Oct 01 00:00:00 UTC 2019

Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures from Adult Mice.

International journal of molecular sciences

Xiao L,Saiki C,Okamura H

14-9118 was used in Immunohistochemistry to study the protective effects of oxidative stress tolerant stem cells harvested from human exfoliated deciduous teeth.

Mon Apr 15 00:00:00 UTC 2019

Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro.

International journal of molecular sciences

Xiao L,Ide R,Saiki C,Kumazawa Y,Okamura H

Published figure using PSA-NCAM monoclonal antibody (Product # 14-9118-82) in Immunofluorescence

Fri Aug 11 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD56; N-CAM-1; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; Polysialylated NCAM; PSA NCAM

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Gene Aliases: CD56; E-NCAM; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC

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UniProt ID: (Human) P13591, (Mouse) P13595, (Rat) P13596

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Entrez Gene ID: (Human) 4684, (Mouse) 17967, (Rat) 24586

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Function(s)

virus receptor activity Ras guanyl-nucleotide exchange factor activity protein binding heparin binding phosphatase binding LRR domain binding identical protein binding fibroblast growth factor receptor binding cytoskeletal protein binding

Process(es)

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway regulation of exocyst assembly cell surface receptor signaling pathway aging learning or memory response to lead ion response to fluoxetine thalamus development neuron projection development multicellular organismal response to stress homotypic cell-cell adhesion response to cocaine neuron development positive regulation of calcium-mediated signaling regulation of sensory perception of pain positive regulation of cardiac muscle cell proliferation negative regulation of cell death response to inorganic substance peripheral nervous system axon regeneration calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules organ regeneration cellular response to molecule of bacterial origin cellular response to inorganic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSA-NCAM Monoclonal Antibody (12E3), eBioscience™

PSA-NCAM Antibody (14-9118-82) in ICC/IF

Product Details

14-9118-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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